湘西龙山百合总黄酮的纯化工艺及抑菌活性

doi:10.13438/j.cnki.jdzk.2024.06.004

吉首大学学报（自然科学版） ›› 2024, Vol. 45 ›› Issue (6): 24-30.DOI: 10.13438/j.cnki.jdzk.2024.06.004

湘西龙山百合总黄酮的纯化工艺及抑菌活性

朱蓝萍，袁姣，袁洁盈，尹欣联，刘英，成江，姚姝凤

（1.吉首大学药学院，湖南吉首 416000;2.武陵山区药用资源化学与药理湖南省高校重点实验室,湖南吉首 416000）

出版日期:2024-11-25 发布日期:2024-12-19
基金资助:
湖南省自然科学基金区域联合基金（2024JJ7409）；吉首大学2021年引进人员资助研究项目；吉首大学药学院创新性研究项目（2022年立项）

Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity

ZHU Lanping,YUAN Jiao,YUAN Jieying,YIN Xinlian,LIU Ying,CHENG Jiang,YAO Shufeng

(1.School of Pharmaceutical Sciences,Jishou University,Jishou 416000,Hunan China;2.Chemical and Pharmacological Laboratory of Medicinal Resources in Wuling Mountainous Area，Jishou University,Jishou 416000,Hunan China)

Online:2024-11-25 Published:2024-12-19

摘要/Abstract

摘要：比较了7种大孔树脂的静态吸附-解吸性能，优选出吸附率和解吸率较高的大孔树脂，利用单因素试验和正交试验筛选出大孔树脂法纯化龙山百合总黄酮的最优工艺，并测定了龙山百合总黄酮纯化前后的总黄酮含量及抑菌活性.结果表明，吸附性能和解吸性能较高的大孔树脂是AB-8；最优纯化工艺为上样液总黄酮质量浓度0.076 4 g/L，上样液pH值4，吸附时间8 h，洗脱液体积分数60%，洗脱液体积120 mL，解吸时间10 h；纯化后的总黄酮质量分数为11.55%，是纯化前的5.3倍；纯化后的总黄酮抑菌圈直径大于纯化前的总黄酮抑菌圈直径，对金黄色葡萄球菌的抑制效应高于对大肠杆菌的抑制效应.

关键词： 龙山百合, 大孔吸附树脂, 总黄酮纯化, 抑菌活性

Abstract: Through comparing the static adsorption-desorption performance of 7 kinds of macroporous resins,the best macroporous resin with high adsorption rate and desorption rate was selected.The optimal purification process of Lilium lancifolium by macroporous resin was selected by single factor experiment and orthogonal experiment,and the total flavonoid content and antibacterial activity before and after purification were determined.The results showed that the macroporous resin with high adsorption and desorption performance was AB-8.The optimal purification process was as follows:the total flavonoid concentration of the feed solution was 0.076 4 g/L,the pH value was 4,the adsorption time was 8 hours,the solvent volume fraction was 60%,the solvent volume was 120 mL,and the desorption time was 10 hours.The total flavonoid content after purification was 11.55%,which was 5.3 times that of the purification before.The inhibition zone diameter of the purified total flavonoid was larger than that of the total flavonoid before purification,and the inhibitory effect on Staphylococcus aureus was higher than that on Escherichia coli.

Key words: Lilium lancifolium thunb, macroporous adsorption resin, total flavone purification, antibacterial activity

朱蓝萍, 袁姣, 袁洁盈, 尹欣联, 刘英, 成江, 姚姝凤. 湘西龙山百合总黄酮的纯化工艺及抑菌活性[J]. 吉首大学学报（自然科学版）, 2024, 45(6): 24-30.

ZHU Lanping, YUAN Jiao, YUAN Jieying, YIN Xinlian, LIU Ying, CHENG Jiang, YAO Shufeng. Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity[J]. Journal of Jishou University(Natural Sciences Edition), 2024, 45(6): 24-30.

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湘西龙山百合总黄酮的纯化工艺及抑菌活性

Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity

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