赤红球菌JDM312株环己酮单加氧酶基因的克隆和序列分析

journal6 ›› 2011, Vol. 32 ›› Issue (3): 82-85.

赤红球菌JDM312株环己酮单加氧酶基因的克隆和序列分析

（吉首大学生物资源与环境科学学院，湖南吉首 416000）

出版日期:2011-05-25 发布日期:2012-04-08
通讯作者: 吾鲁木汗·那孜尔别克(1961-)，女，新疆塔城托里人，吉首大学生物资源与环境科学学院教授，博士，主要从事微生物学研究.E-mail:ulum@jsu.edu.cn.
基金资助:
湖南省大学生研究性学习和创新性实验计划项目(No.CX2008146)

Cloning and Sequencing of Cyclohexanone Monooxynase Gene from Rhodococcus ruber JDM312

(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,China)

Online:2011-05-25 Published:2012-04-08

摘要/Abstract

摘要：应用PCR从赤红球菌JDM312株基因组DNA中扩增出chnB基因序列，构建pUC18-chnB重组质粒，转化大肠杆菌DH5α，并对插入片段进行测序，用Clustal X 和Mega 3.1 软件对测定DNA序列进行相似性和系统发育分析.结果显示：扩增得到的chnB基因大小为1 623 bp，编码由542个氨基酸残基构成的多肽，与已报道不同细菌属菌种chnB基因核苷酸序列的相似性为85%~98%，其中与红球菌Phi2株的亲缘关系最近.

关键词： 赤红球菌, 环己酮降单加氧酶, 克隆, 序列分析

Abstract: The chnB gene encoding a cyclohexanone monooxygenase was amplified by PCR from genomic DNA of Rhodococcus ruber JDM312,the PCR product was cloned into the pUC18 vector and sequenced,and the sequence identity searches were performed by using software Clustal X and Mega 3.1.The sequence analysis showed that the open reading frame of the chnB gene from Rhodococcus ruber JDM312 was 1 623 bp and codes for a polypeptide of 542 amino acids.DNA identities of the chnB genes between the JDM312 strain and the previously reported four other stains of Rhodococcus was 85% to 98%,and the phylogenic tree showed that these strains clustered one main branch.It can be concluded that the chnB gene of Rhodococcus ruber JDM312 is useful to further study on expression of cyclohexanone monooxygenase and its enzymatic activity.

Key words: rhodococcus ruber；cyclohexanone monooxygenase；cloning , sequencing

彭哲慧, 许大奎, 王静芳, 吾鲁木汗.孜尔别克. 赤红球菌JDM312株环己酮单加氧酶基因的克隆和序列分析[J]. journal6, 2011, 32(3): 82-85.

PENG Zhe-Hui, XU Da-Kui, WANG Jing-Fang, WU Lu-Mu-Han-.Zi-尔Bie-Ke. Cloning and Sequencing of Cyclohexanone Monooxynase Gene from Rhodococcus ruber JDM312[J]. journal6, 2011, 32(3): 82-85.

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