牛血清白蛋白修饰水溶性CdTe量子点及分析应用

journal6 ›› 2011, Vol. 32 ›› Issue (1): 93-97.

牛血清白蛋白修饰水溶性CdTe量子点及分析应用

(吉首大学化学化工学院，湖南吉首 416000)

出版日期:2011-01-25 发布日期:2012-04-10
通讯作者: 陈莉华（1961-),女,教授,博士,主要从事纳米粒子的生物应用研究.E-mail：chenlihua99@163.com.
作者简介:张文龙（1987-),男,山西运城人,吉首大学化学化工学院硕士研究生,主要从事纳米粒子的生物应用研究
基金资助:
南京大学生命分析化学教育部重点实验室开放基金资助项目（KLACLS1003）

Surface-Modification of CdTe Quantum Dots with Bovine Serum Albumin and Analytical Application

(College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China)

Online:2011-01-25 Published:2012-04-10

摘要/Abstract

摘要：以巯基乙酸为稳定剂，采用水热法合成了CdTe 量子点，用牛血清白蛋白改变量子点的表面修饰状态并通过荧光发射光谱研究了溶液pH、温度和离子强度对表面修饰产生的影响.结果表明，CdTe量子点在502 nm处有吸收，在538 nm处有荧光发射，经牛血清白蛋白对其表面修饰后，吸收峰位不变，但吸光强度升高；荧光发射峰位不变，荧光显著增强.在优化的反应条件下，牛血清白蛋白质量浓度在0.05~1.0 mg/L范围内与荧光增加值呈线性关系，检出限为0.011 7 mg/L。以缓冲溶液为基底牛血清白蛋白含量测定获得满意结果.

关键词： CdTe, 量子点, 牛血清白蛋白, 表面修饰

Abstract: Mercaptoacetic acid-stabilized CdTe quantum dots were prepared by hydrothermal synthesis method.The CdTe quantum dots exhibited absorption at 502 nm and fluorescence emission at 538 nm,respectively.When surface-modification of CdTe quantum dots were replaced with BSA,absorption peak and the fluorescence peak remained the same,while absorption intensity and the fluorescence emission intensity increased.The effect of pH、temperature and ion strength on fluorescence intensity were discussed.Under the optimal conditions,a mass concentration of BSA in the range of 0.05~1.0 mg/L shows a linear relationship with the fluorescence intensity increasing value of CdTe quantum dots,with a detection limit of 0.011 7 mg/L.The proposed method was applied to detection of the concentration of BSA in buffer solution.

Key words: CdTe, quantum dots, bovine serum albumin, surface-modification

张文龙, 张俊生, 周丽平, 陈莉华. 牛血清白蛋白修饰水溶性CdTe量子点及分析应用[J]. journal6, 2011, 32(1): 93-97.

ZHANG Wen-Long, ZHANG Jun-Sheng, ZHOU Li-Ping, CHEN Li-Hua. Surface-Modification of CdTe Quantum Dots with Bovine Serum Albumin and Analytical Application[J]. journal6, 2011, 32(1): 93-97.

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