实时荧光反转录PCR检测呼吸道合胞病毒

journal6 ›› 2010, Vol. 31 ›› Issue (5): 101-103.

实时荧光反转录PCR检测呼吸道合胞病毒

（1.益阳医学高等专科学校，湖南益阳 413000；2.深圳市第四医院，广东深圳 518000）

出版日期:2010-09-25 发布日期:2012-04-12
作者简介:陈明雄（1961-），男，湖南临澧人，益阳医学高等专科学校副教授，硕士，主要从事分子生物学及免疫学研究.

Detection of Respiratory Syncytial Virus by Real Time Reverse Transcription PCR

(1.Yiyang Medical College,Yiyang 413000,Hunan China;2.Shenzhen Fourth Hospital,Shenzhen 518000,Guangdong China)

Online:2010-09-25 Published:2012-04-12

摘要/Abstract

摘要：目的建立快速、准确、特异的实时荧光反转录PCR方法检测呼吸道合胞病毒(RSV).方法根据RSV基因序列设计引物和探针并优化实时荧光反转录PCR反应体系，对686例临床标本进行检测，阳性结果测序验证.结果本实验所建立实时荧光反转录PCR方法可准确、特异地检测RSV；686名呼吸道感染患儿中RSV感染达到16.5%(113/686).结论本研究建立的RSV实时荧光反转录PCR方法快速、准确，结果可靠，可用于儿童RSV感染的临床诊断.

关键词： 实时荧光反转录PCR, 呼吸道合胞病毒, 检测方法

Abstract: Objective To develop a rapid,sensitive and specific real-time reverse transcription PCR(RT-PCR) for detecting respiratory syncytial virus (RSV).Method The primer and probe were designed according to the RSV gene sequency,and the real-time reverse transcription PCR reaction was optimized.The real time RT-PCR was applied to detect 686 clinical respiratory specimens.Results The RSV was detected by real time RT-PCR accurately and quickly.Among those 686 specimens,113(16.5%) were positive for RSV.Conclusion Real time RT-PCR is a reliable and accurate assay for RSV.The detecting method can be applied to the clinical diagnosis of the RSV infection in children.

Key words: real time reverse transcription PCR, respiratory virus

陈明雄, 郭劲霞, 杜兆丰, 欧阳方丹, 黄烈, 陆学东, 曾凡胜. 实时荧光反转录PCR检测呼吸道合胞病毒[J]. journal6, 2010, 31(5): 101-103.

CHEN Ming-Xiong, GUO Jin-Xia, DU Zhao-Feng, OuYang-Fang-Dan , HUANG Lie, LU Xue-Dong, ZENG Fan-Sheng. Detection of Respiratory Syncytial Virus by Real Time Reverse Transcription PCR[J]. journal6, 2010, 31(5): 101-103.