蛇足石杉外植体表面消毒及内生菌消除方法

journal6 ›› 2009, Vol. 30 ›› Issue (4): 100-103.

蛇足石杉外植体表面消毒及内生菌消除方法

(1,吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首 416000;2.中国科学院华南植物园,广东广州 510650)

出版日期:2009-07-25 发布日期:2012-04-22
通讯作者: 李菁，吉首大学教授，E-mail lkg@jsu.edu.cn.
作者简介:李贵（1982-），男,湖南桃江人,吉首大学植物资源保护与利用湖南省高校重点实验室硕士研究生,主要从事植物生态学研究
基金资助:
湖南省科学技术厅科技计划（2007NK3075）；广东省科技计划资助项目（2007B020704003）

Sterilization of Explants and Elimination of Endophytes in Huperzia Serrata

(1.Key Laboratory of Plant Resources Conservation and Utilization,Jishou University,Jishou 416000,Hunan China;2.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China)

Online:2009-07-25 Published:2012-04-22

摘要/Abstract

摘要：为了获得蛇足石杉的无菌材料，使用双氧水、酒精、升汞等消毒剂对外植体进行表面消毒，使用混合抗菌溶液及不同温度的热水浴处理外植体以消除内生菌.结果表明:茎段经预处理后，用47 ℃热水浴处理1 h，再经70 %酒精处理30 s, 0.1 %升汞处理7 min后，能达到较好的表面消毒及内生菌消除的效果；孢子囊和茎尖经预处理后，再经过70%酒精处理30 s,0.1 %升汞处理4 min即可达到较好的表面消毒效果；消毒剂结合温度处理能降低内生菌的污染率；孢子囊和茎尖几乎不含内生菌，是可用于组织培养的较好的外植体.

关键词： 蛇足石杉, 外植体, 表面消毒, 内生菌

Abstract: In order to obtain sterile materails from Huperzia serrata,H2O2,C2H5OH,and HgCl2 were used to disinfect the explants,and the solutions of antibiotic compound together with hot water treatments were used to eliminate the endophytes.The results were as follows:the optimum method of the sterilization of surface and elimination of endophytes for stem was obtained by soaking the explants in hot water (47 ℃) for 1 h,followed by 70% C2H5OH for 30 s and then by 0.1 % HgCl2 for 7 min.The optimum method of the sterilization of surface for sporangium and stem apex was obtained by soaking the explants in 70 % C2H5OH for 30 s,followed by 0.1% HgCl2 for 4 min.Disinfectants together with hot water treatment can obviously reduce the pollution rate of explants.Sporangium and stem apex,which barely contain endophytes,sre excellent explants for plant tissue culture.

Key words: Huperzia serrata, explants, sterilization, endophytes

李贵, 李菁, 黎有有, 唐源江, 魏华. 蛇足石杉外植体表面消毒及内生菌消除方法[J]. journal6, 2009, 30(4): 100-103.

LI Gui, LI Jing, LI You-You, TANG Yuan-Jiang, WEI Hua. Sterilization of Explants and Elimination of Endophytes in Huperzia Serrata[J]. journal6, 2009, 30(4): 100-103.

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