酶解法预处理杜仲粕壳考察指标的研究

journal6 ›› 2009, Vol. 30 ›› Issue (2): 74-77.

酶解法预处理杜仲粕壳考察指标的研究

（1.湖南省林产化工工程重点实验室，湖南张家界 427000；2.湖南中医药大学，湖南长沙 410007 ）

出版日期:2009-03-25 发布日期:2012-04-25
通讯作者: 张永康，男，教授，博士生导师.
作者简介:刘贵华（1982-），男，湖南邵阳人，硕士研究生，主要从事天然产物提取分离及加工研究.
基金资助:
湖南省经贸委科技攻关重点项目《杜仲果实综合开发利用》[2004（307）]

Research of Lnvestigation Targets in Enzyme Pretreatment Shell of Eucommia Ulmoide

(1.Key Lab.of Forest Products and Chemical Engineering，Jishou University，Zhangjiajie 427000，Hunan China;2.TCM University of Hunan，Changsha 410007，China)

Online:2009-03-25 Published:2012-04-25

摘要/Abstract

摘要：为探讨杜仲粕壳酶解液中总糖含量的测定方法的可行性，将其总糖含量作为酶解率的考察指标，在相同酶解条件下，同一用量的不同种类酶和不同用量的同一种酶分别对杜仲粕壳酶解，采用蒽酮比色法，以供试品空白为参比测定酶解液中总糖的含量.结果表明:在625 nm波长下测得的各酶解液中总糖含量能较好地反应出酶解率的不同，同时酶解液中的酶不会影响蒽酮比色法测定酶解总糖的含量作为酶解率的考察指标.采用差示光度法(△A法)可消除酶解液中干扰测定总糖含量的许多因素，方法简单，结果准确，可为研究酶解法预处理样品原料的酶解率提供一种可行的考察指标.

关键词： 杜仲粕壳, 总糖含量, 蒽酮比色法, 酶解法, 考察指标, 差示光度法

Abstract: Objective To explore whether the method of determination of the total sugar in enzyme solution of eucommial seed shell can be feasible.Mothods The total sugar content was taken as the study indicators of digestion rate.In the same conditions，eucommial seed shell was degraded by the same amount of different types of enzymes and by the different amounts of an enzyme respectively.When sample was taken as blank fiduciary the total sugar was determined with anthrone colorimetry.Results The measured total sugar of the enzyme solution better reflected the different rates of digestion in the 625 nm wavelength,and the total sugar determined with anthrone colorimetry and total sugar content as an indicator of the enzymes rate will not be affected by enzyme in enzyme solution of eucommial seed shell.Conclusions The method can eliminate interfering factor of the enzyme solution with differential spectrophotometry (△A method),which can prove a simple and accurate method for an indicator of the enzymes rate in the pretreatment samples of raw materials.

Key words: shell of eucommia ulmoide, total sugar content, anthrone colorimetry, enzymatic method, investigation targets；differential spectrophotometry

刘贵华, 肖美凤, 彭新君, 张永康. 酶解法预处理杜仲粕壳考察指标的研究[J]. journal6, 2009, 30(2): 74-77.

LIU Gui-Hua, XIAO Mei-Feng, PENG Xin-Jun, ZHANG Yong-Kang. Research of Lnvestigation Targets in Enzyme Pretreatment Shell of Eucommia Ulmoide[J]. journal6, 2009, 30(2): 74-77.