原儿茶酸分子印迹聚合物的色谱识别性能

journal6 ›› 2008, Vol. 29 ›› Issue (4): 75-82.

原儿茶酸分子印迹聚合物的色谱识别性能

（1．吉首大学林产化工工程省级重点实验室，湖南张家界 427000；2．吉首大学化学化工学院，湖南吉首 416000）

出版日期:2008-07-25 发布日期:2012-05-21

Analogue Recognition and Chromatographic Behaviors of MolecularlyImprinted Polymer for Protocatechuic Acid

(1.Hunan Province Key Laboratory of Forstry Products & Chemical Engineering,Jishou University,Zhangjiajie 427000,Hunan China;2.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China)

Online:2008-07-25 Published:2012-05-21
About author:Correspondence author,LI Hui(1971-),male,was born in Cili,Hunan,master,associate professor;research area is the new analytical method in lifescience.E-mail:lihuijsdx@163.com.
Supported by:
Science Research Foundation of Administration of Education of Hunan Province

摘要/Abstract

摘要：应用非共价法在四氢呋喃溶剂中合成了原儿茶酸印迹聚合物.通过静态平衡吸附实验和高效液相色谱分析，评价了分子印迹聚合物对模板及其类似物的键合行为.结果表明：由于分子印迹聚合物基体存在大量多空的识别位点，并且这些位点的大小和形状与模板分子十分匹配，因此，分子印迹聚合物对模板分子具有高的吸附能力和选择性.Scatchard分析表明印迹聚合物表面有2类不同性能的键合位点.流动相及其流速对分析物的保留行为有较大影响.

关键词： 分子印迹聚合物, 原儿茶酸, 平衡吸附, 高效液相色谱

Abstract: A non-covalent imprinting approach was applied to synthesize protocatechuic acid (3,4-dihydroxybenzoic acid) imprinted polymers (MIPs) in the polar tetrahydrofuran by using methacrylic acid as functional monomer.By equilibrium adsorption experiments and high performance liquid chromatography (HPLC),the rebinding properties of the MIPs to protocatechuic acid (template) and its analogues were evaluated.It was found that the MIPs exhibited high adsorption capability and selectivity for the template and two kinds of absorption sites in the polymer were detected by Scatchard curve analysis.The eluents and the mobile phase flow rate had an important influence on the retention of the analytes on the MIPs column.All of the results showed that the stronger affinity of the MIPs to the template than its analogues resulted from the size and shape recognition in the MIPs network based on hydrogen bonding interaction.

Key words: molecularly imprinted polymer, protocatechuic acid, equilibrium adsorption, HPLC

李亚男, 李辉, 石慧, 张朝晖, 胡文斌, 李志平. 原儿茶酸分子印迹聚合物的色谱识别性能[J]. journal6, 2008, 29(4): 75-82.

LI Ya-Nan, LI Hui, SHI Hui, ZHANG Chao-Hui, HU Wen-Bin, LI Zhi-Ping. Analogue Recognition and Chromatographic Behaviors of MolecularlyImprinted Polymer for Protocatechuic Acid[J]. journal6, 2008, 29(4): 75-82.

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