生殖域中特异表达的新基因Rcet2的克隆

journal6 ›› 2008, Vol. 29 ›› Issue (3): 105-108.

生殖域中特异表达的新基因Rcet2的克隆

(1.南方医科大学生物技术学院，广东广州510515；2.宁波东方红航天生物科技开发有限公司，浙江宁波 315336；3.湖南理工学院，湖南岳阳 414000)

出版日期:2008-05-25 发布日期:2012-05-23
通讯作者: 周明乾，男，实验师，E-mail:zmp12@163.com;聂东宋(1965-)，男，博士，副教授，E-mail：ndz1997@126.com.
作者简介:向阳(1968-)，男，博士，南方医科大学生物技术学院副教授，主要从事分子生物学研究

Cloning of New Gene Rcet2 Expressed Specifically in Reproduction Tract

(1.College of Biotechnology,Southern Medical University,Guangzhou 510515,China；2.Ningbo Dongfanghong Co. Ltd. of Aerospace Biological Technology Development，Ningbo 315336,Zhejiang China；3.Hunan Institute of Science and Technology,Yueyang 414000,Hunan China)

Online:2008-05-25 Published:2012-05-23

摘要/Abstract

摘要：利用NCBI中的数字差异显示数据库首先电子克隆了Rcet2基因，然后从成年小鼠睾丸组织中克隆出Rcet2全长基因，序列分析显示：Rcet2基因全长cDNA为454 bp，含3个外显子，编码88个氨基酸残基，该蛋白含1个不完整的半胱氨酸蛋白酶抑制剂结构域，但缺少半胱氨酸蛋白酶抑制剂关键的作用位点，而这些位点对半胱氨酸蛋白水解酶的抑制作用是重要的.小鼠多组织RT-PCR结果显示，Rcet2在成年老鼠大脑、睾丸和附睾生殖域中特异表达，睾丸中的表达高于附睾和大脑，Rcet2与半胱氨酸蛋白酶抑制家族2的Cres亚家族具有相似的特征，结果显示Rcet2可能是Cres亚家族中的一个新成员.

关键词： Rcet2 , 克隆, 生殖域, 半胱氨酸蛋白酶抑制剂

Abstract: The authors used digital differential display data in NCBI to clone Rcet2 which was isolated from adult mouse testis cDNA library.Sequence analysis revealed that Rcet2 cDNA was 454 bp in length,and had three exons,with the result that these cDNAs encoded one protein.The deduced protein was 88 residues in length and had one potential signal peptide and one cystatin domain,but lacked part critical consensus sites important for cysteine protease inhibition.These characteristics are seen in Cres subgroup,which is related to the family 2 cystatains.RT-PCR analysis showed that Rcet2 was specifically expressed in adult mouse testis,epididymis and cerebrum,but higher in testis than in epididymis and cerebrum.This Rcet2 may be new members of Cres subgroup of family 2 cystatins.

Key words: Rcet2, new gene, reproduction tract, cystatains

向阳, 邹寿长, 周延凯, 周明乾, 聂东宋. 生殖域中特异表达的新基因Rcet2的克隆[J]. journal6, 2008, 29(3): 105-108.

XIANG Yang, ZOU Shou-Chang, ZHOU Yan-Kai, ZHOU Ming-Qian, NIE Dong-Song. Cloning of New Gene Rcet2 Expressed Specifically in Reproduction Tract[J]. journal6, 2008, 29(3): 105-108.

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