反相高效液相法测定八角茴香果实中的莽草酸

journal6 ›› 2008, Vol. 29 ›› Issue (1): 104-106.

反相高效液相法测定八角茴香果实中的莽草酸

(1.吉首大学生物资源与环境科学学院，湖南吉首 416000；2.湖南省林产化工重点实验室，湖南张家界 427000；3.华南农业大学食品科学学院，广东广州 510642)

出版日期:2008-01-25 发布日期:2012-05-26
通讯作者: 曹庸，教授，博士生导师.
作者简介:李伟（1982－），男，云南曲靖人，硕士生，主要从事农业资源与应用方面的研究
基金资助:
湖南省科技厅重点资助项目（06FJ4046）

Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F.

(1.College of Biology and Environmental Science,Jishou University,Jishou,Hunan 416000,China;2.Key Laboratory of Hunan Forest Products and Chemical Industry Engineering,Zhangjiajie Campus of Jishou University,Zhangjiajie Hunan 427000,China;3.College of Food Science,South China Agriculture University,Guang zhou 510642,China)

Online:2008-01-25 Published:2012-05-26

摘要/Abstract

摘要：用反相高效液相法在Waters Symmetry C18柱(5 μm,4.6 mm×150 mm i.d)上测定八角茴香果实中的莽草酸.工作参数为：流速0.5 mL/min；进样体积2 μL；检测波长220 nm；柱温30 ℃±1 ℃；流动相0.01 mol/L　K2HPO4磷酸盐缓冲液(pH值为3，用磷酸调配)和体积分数3%甲醇.平均回收率为97.76 %，RSD为1.937 %(n=5).

关键词： 反相高效液相色谱法, 莽草酸, 八角茴香

Abstract: OBJECTIVE To establish a RP－HPLC method for the determination of Shikimic Acid in Illicium verum Hook.f.METHODS In RP－HPLC analysis,a Waters Symmetry C18 column (5 μm,4.6 mm×150 mm,i.d.) was selected as separation column at 30 ℃±1 ℃.The mobile phase consisted of 0.01 mol/L K2HPO4 phosphate buffer (pH=3,I =0.17 mol/L) and 3% methanol.The flow rate was 0.5 mL/min and the wavelength of measurement was 220 nm.The average recovery was 97.76% with RSD of 1.937% (n=5).CONCLUSION The method was proved to be quick,simple,and highly sensitive and can be useful in Shikimic Acid medicines quality control.

Key words: RP－HPLC, Illicium verum Hook.f., Shikimic Acid

李伟, 曹庸, 魏华, 周冬武. 反相高效液相法测定八角茴香果实中的莽草酸[J]. journal6, 2008, 29(1): 104-106.

LI Wei, CAO Yong, WEI Hua, ZHOU Dong-Wu. Reversed-Phase HPLC Determination of Shikimic Acid in Illicium Verum Hook.F.[J]. journal6, 2008, 29(1): 104-106.