原发性肝癌组织中TAM和MC与MV计数及病理意义

journal6 ›› 2007, Vol. 28 ›› Issue (5): 120-124.

原发性肝癌组织中TAM和MC与MV计数及病理意义

（1.吉首大学医学院湖南吉首 416000；2.中南大学湘雅二医院肝胆疾病研究室，湖南长沙 410011）

出版日期:2007-09-25 发布日期:2012-06-11
作者简介:向志钢（1969-），男(土家族)，湖南凤凰人，吉首大学医学院副教授，硕士，主要从事外科学教学及临床肝胆胰疾病研究.
基金资助:
湖南省教育厅科学研究项目(05C136)

Count of TAM,MC,MV in Tissues of Primary Hepatocarcinoma and Its Clinical Significance

(1.Medical College of Jishou University,Jishou 416000,Hunan China;2.Laboratory of Hepatobiliary Diseases,Xiangya Second Hospital of Central South University,Changsha 410011,China)

Online:2007-09-25 Published:2012-06-11

摘要/Abstract

摘要：目的研究原发性肝癌（primary hepatocarcinoma,PHC）组织中肿瘤相关巨噬细胞（tumor-associated macrophage,TAM）、肥大细胞（mast cells,MC）和微血管（microvessel,MV）的计数及其临床病理意义，并探讨了三者之间的相互关系.方法应用ABC免疫组化法，对47例PHC手术切除标本常规石蜡包埋切片分别检测TAM、MC和MV，并高倍镜下计数.结果 47例PHC癌组织中TAM、MC和MV计数均显著高于癌旁组织（TAM：70.27±17.93个/HP vs. 44.15±9.10个/HP，P<0.01；MC：15.46±4.54个/HP vs. 8.08±2.03个/HP，P<0.01；MV：67.30±13.68个/HP vs. 37.20±10.58个/HP，P<0.01）；TAM与MV在术前AFU（α-岩藻糖）小于等于10 μg/L病例的计数显著高于AFU大于10 μg/L病例的计数(TAM：74.13±18.33个/HP vs. 61.15±13.54个/HP,P<0.05;MV：70.41±13.03个/HP vs. 59.97±12.69个/HP,P<0.05)；伴转移病例癌组织中的MV计数（73.50±13.77个/HP）显著高于无转移病例(64.10±12.68个/HP，P<0.05)；MC、TAM、MV在PHC中的计数与PHC其他主要临床病理特征之间均无明显关系；癌组织中TAM计数与MV计数呈正相关（P<0.01，r=0.686），MC计数与MV计数呈正相关（P<0.05，r=0.348）.结论 MC、TAM、MV的计数与PHC 的发生、发展关系密切；MV计数高的PHC易于发生浸润和转移；TAM、MC与PHC的血管生成可能有密切的关系.

关键词： 原发性肝癌, 微血管, 巨噬细胞, 肥大细胞, 免疫组织化学

Abstract: Objective To study the count of TAM,MC and MV in tissues of PHC and detect the relation to the clinicopathological features of PHC,and the relation among the count of TAM,MC and MV.Methods The stains of TAM,MC and MV were completed by the immunohistochemical method of avidin-biotin complex in routine paraffin-embedded sections from 47 cases with PHC.Results The count means of TAM,MC and MV in the cancer tissues were significantly higher than that in the peri-cancerous tissues (TAM：70.27±17.93/HP vs. 44.15±9.10/HP,P<0.01；MC：15.46±4.54/HP vs. 8.08±2.03 /HP，P<0.01；MV：67.30±13.68/HP vs. 37.20±10.58/HP,P<0.01);except the count of TAM and MV in the patients with AFU (pre-operation)≤10 μg/L in serum were significantly higher than that whose AFU>10 μg/L (TAM:74.13±18.33/HP vs. 61.15±13.54/HP,P<0.05;MV：70.41±13.03/HP vs. 59.97±12.69/HP,P<0.05);the count of MV in the patients with metastasis (73.50±13.77/HP) was significantly higher than that of non-metastasis (64.10±12.68/HP,P<0.05);there were no correlations among the count of MV,TAM and the other clinicopathological features (including pathological type,differentiated degree,the value of AFP,with or without SHCSP,cirrhosis,cancer emboli,gross shapes,and maximal diameter of mass,etc).The correlation analysis shows that the count of TAM was significantly correlated with the count of MV （r=0.686，P<0.01）,and the count of MC was significantly correlated with the count of MV （r=0.348，P<0.05）.Conclusions The count of MC,TAM and MV might be closely related to the carcinogenesis and the progress of PHC;the count of MV might be associated with tumor invasion and metastasis;TAM and MC might be closely related to the tumor angiogenesis.

Key words: primary hepatorcacinoma, microvessel, macrophage；mast cell；immunohistochemistry

向志钢, 杨竹林, 钟飞. 原发性肝癌组织中TAM和MC与MV计数及病理意义[J]. journal6, 2007, 28(5): 120-124.

XIANG Zhi-Gang, YANG Zhu-Lin, ZHONG Fei. Count of TAM,MC,MV in Tissues of Primary Hepatocarcinoma and Its Clinical Significance[J]. journal6, 2007, 28(5): 120-124.

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