journal6 ›› 2007, Vol. 28 ›› Issue (3): 95-98.

• 生物资源 • 上一篇    下一篇

转乙肝病毒表面抗原基因烟草发根的获得

  

  1. (福建师范大学生命科学学院,发育与神经生物学福建省高校重点实验室,福建  福州 350108)
  • 出版日期:2007-05-25 发布日期:2012-08-27
  • 作者简介:王逸群(1964-),男,博士,福建师范大学生命科学学院,发育与神经生物学福建省高校重点实验室副教授,主要从事植物分子生物学研究.
  • 基金资助:

    福建省自然科学基金资助项目(B0410009);福建省科技厅资助项目(2004N026);福建省教育厅科学研究项目(JB03125)

Transformation of  HBsAg Gene into Tobacco  and  Production of Transgenic Tobacco Hairy Roots

  1. (The Key Lab of Developmental and Neural Biology in Fujian Provincial Universities,College of Life Sciences,Fujian Normal University,Fujian 350108,China)
  • Online:2007-05-25 Published:2012-08-27

摘要:构建了乙肝病毒表面抗原基因(HBsAg)植物表达载体,通过冻融法将HBsAg 基因转入到发根农杆菌LBA1314中,采用叶盘法将HBsAg基因导入到烟草中,获得了转基因烟草发根.对转基因烟草发根的GUS检测结果表明:转HBsAg基因烟草发根可以染成蓝色,而非转基因烟草的根没有染色反应.这说明gus基因在转HBsAg基因烟草发根获得了表达.

关键词: 乙肝病毒表面抗原基因, 烟草, 发根, 转化

Abstract: A plant expression carrier containing HBsAg (hepatitis B virus surface antigen) gene was constructed in a plasmid,the plasmid was mobilized into a rhizogenes Strain LBA 1314 by freeze-thaw method,the HBsAg gene was planted into tobacco by leaf disc via Agrobacterium tumefaciens LBA 4404 as a vehicle and the tobacco hairy roots were received.Histochemical staining of GUS activity was made in the transgenic tobacco hairy roots:The roots with HBsAg genes can be dyed into bule,while those without have no reflection of color.So the conclusion can acquire that the gene has its expression in the roots.

Key words: hepatitis B virus surface antigen gene, tobacco, hairy roots, transformation

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