二氢杨梅素印迹聚合物的表面行为

journal6 ›› 2006, Vol. 27 ›› Issue (6): 103-106.

二氢杨梅素印迹聚合物的表面行为

（1.湖南省林产化工工程重点实验室，湖南张家界 427000；2.吉首大学化学化工学院，湖南吉首 416000；3.湖南大学化学化工学院，湖南长沙 410082）

出版日期:2006-11-25 发布日期:2012-06-28
作者简介:李辉(1971-),男（土家族），湖南慈利人，湖南省林产化工工程重点实验室副教授,博士,主要从事生命科学中的分离分析新方法研究.
基金资助:
国家自然科学基金资助项目(20275010)

Investigation on the Surface Behavior of Dihydromyricetin Imprinted Polymer Monolith

(1.Hunan Key Laboratory of Chemical Engineering of Forest Products,Zhangjiajie 427000,Hunan China;2.College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,Hunan China；3.State Key Laboratory of Chemo/Biological Sensing & Chemometrics,Hunan University,Changsha 410082,China)

Online:2006-11-25 Published:2012-06-28

摘要/Abstract

摘要：应用前沿分析技术测定了二氢杨梅素印迹聚合物块的实验吸附等温线，并用Langmuir方程、Bi-Langmuir方程和Freundlich方程进行拟合，从最佳拟合求得了印迹聚合物的表面位点分布、吸附常数和饱和吸附量.测定了不同温度和不同流动相组成时分子印迹聚合物固定相的吸附等温线.结果表明：双朗格缪尔模型和福伦登里西模型均能给出较好的拟合效果.当20 ℃、四氢呋喃为流动相时，从Bi-Langmuir方程的最佳拟合参数得到第1类键合位点的键合常数和饱和吸附量分别为0.39 mL·mg^-1和133.23 mg·mL^-1，第2类键合位点的键合常数和饱和吸附量分别是135.07 mL·mg^-1和0.071 mg·mL^-1.

关键词： 前沿分析技术, 分子印迹聚合物块, 二氢杨梅素, 吸附等温线, 分布位点

Abstract: Determination of adsorption isotherm of dihydromyricetin imprinted polymer monolith was carried out by frontal analysis technique.Langmuir,Bi-Langmuir and Freundlich equations were used for the fit of these isotherms in order to obtain the site distribution,binding constants and saturation capacities of the polymer monolith at different temperatures and under different mobile phase make-up,respectively.The results indicated that the fittings of the data to Bi-Langmuir and Freundlich model were both good.From the best fitting of Bi-Langmuir equation,it was obtained that the binding constants and saturation capacities at 20 ℃ using tetrahydrofuran as mobile phase were 0.39 mL/mg and 133.23 mg/mL for the first site class and 135.07 mL/mg and 0.071 mg/mL for the second site class,respectively.

Key words: frontal analysis, molecularly imprinted polymer monolith, dihydromyricetin, adsorption isotherm, sites distribution

李辉, 李亚男, 张朝辉, 聂利华, 欧阳玉祝, 李志平. 二氢杨梅素印迹聚合物的表面行为[J]. journal6, 2006, 27(6): 103-106.

LI Hui, LI Ya-Nan, ZHANG Chao-Hui, NIE Li-Hua, OUYang-Yu-Zhu , LI Zhi-Ping. Investigation on the Surface Behavior of Dihydromyricetin Imprinted Polymer Monolith[J]. journal6, 2006, 27(6): 103-106.

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