棘球蚴抗原纯化对免疫球蛋白IgG免疫应答的影响

journal6 ›› 2005, Vol. 26 ›› Issue (4): 29-33.

棘球蚴抗原纯化对免疫球蛋白IgG免疫应答的影响

(北京师范大学生命科学学院,北京100875)

出版日期:2005-10-15 发布日期:2012-09-17
作者简介:郑光宇(1944-)，男，广西柳州人，北京师范大学生命科学学院教授，主要从事微生物生物化学研究.
基金资助:
国家自然科学基金资助项目(3861166)

Effect of Purification of Echinococcus Antigen on Immune Response with Specific Immunoglobulin G

(College of Life Sciences,Beijing Normal University 100875,Beijing China)

Online:2005-10-15 Published:2012-09-17

摘要/Abstract

摘要：以纯化的棘球蚴抗原和未经纯化的粗抗原用于棘球蚴感染者血清中抗棘球蚴抗原的特异性免疫球蛋白IgG水平的检测试验,结果表明,当血清稀释度为1∶200时,使用纯化抗原可以获得较高的特异性 (98.9%),并可保持在使用粗抗原及血清稀释度为1∶400时获得的较高的敏感性（93.7%）,提示在棘球蚴感染者血清中抗棘球蚴抗原特异性免疫球蛋白IgG水平的检测中,使用纯化棘球蚴抗原和粗抗原均可获得较高的敏感性(93.7%)和较高的特异性 (98.4%,98.9%),使用纯化抗原时的特异性略高,但在统计学上并无显著差异.

关键词： 棘球蚴病, 棘球蚴抗原, 免疫球蛋白IgG, 免疫应答, 斑点免疫结合试验

Abstract: The level of specific immunoglobulin G (IgG) in sera of hydatidosis patients against Echinococcus granulosus antigen is detected in dot-immunobinding assay with purified and crude Echinococcus granulosus antigens.The experiment carried out with purified antigen and the serum 1∶200 obtains higher specificity (98.9%);meanwhile,the experiment maintains the higher sensitivity (93.7%) which is obtained in the experiment carried out with crude antigen and the serum dilution 1∶400.These results indicate that in the research of specific immunoglobulin G in sera of hydatidosis patients anti-Echinococcus granulosus antigen,higher sensitivity (93.7%) and higher specificity (98.4,98.9%) can be obtained with purified Echinococcus granulosus antigen and crude Echinococcus granulosus antigen respectively;higher specificity is achieved when purified antigen is used,but there is no significant difference between purified and crude antigen in statistics.

Key words: hydatidosis;echinococcus granulosus antigen;immunoglobulin G;immune , response;dot immunobinding assay

郑光宇, 赵荣乐. 棘球蚴抗原纯化对免疫球蛋白IgG免疫应答的影响[J]. journal6, 2005, 26(4): 29-33.

ZHENG Guang-Yu, ZHAO Rong-Le. Effect of Purification of Echinococcus Antigen on Immune Response with Specific Immunoglobulin G[J]. journal6, 2005, 26(4): 29-33.