生物膜活性测定中TTC-脱氢酶活性测定法的改进

journal6 ›› 2005, Vol. 26 ›› Issue (1): 37-39.

生物膜活性测定中TTC-脱氢酶活性测定法的改进

(1.中国科学院水生生物研究所淡水生态和生物技术国家重点实验室，湖北武汉 430072；2.中国科学院研究生院，北京100039；3.湖北师范学院生物系，湖北黄石 435002)

出版日期:2005-01-15 发布日期:2012-09-22
作者简介:李今（1968-），男，湖南省衡阳市人，湖北师范学院生物系副教授，博士，主要从事环境微生物研究.
基金资助:
国家“十五”重大科技专项资助项目（2002AA601021）;湖北省教育厅重点资助项目(2002A00010)

Improvement of Determination on TTC-Dehydrogenase Activity in Biofilms

(1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,Hubei China；2.Graduate School of the Chinese Academy of Sciences,Beijing 100039;3.Biology Department,Hubei Normal University,Huangshi 435002,Hubei China)

Online:2005-01-15 Published:2012-09-22

摘要/Abstract

摘要：对生物膜活性测定中氯化三苯基四氮唑(TTC)-脱氢酶活性测定法进行了改进，解决了标准曲线制作稳定性差的问题，对测定中的诸多影响因素进行比较分析，确定了改进后的脱氢酶活性测定的最佳条件.结果表明，以甲苯作为萃取剂的液-液分层明显，提取效果好，操作简便.以硫化钠代替连二亚硫酸钠作还原剂，效果较好，显色稳定不褪色.反应的适宜pH值为8.6，适宜温度为38 ℃.同时确定了生物膜的最佳培养反应时间为6 h.

关键词： 生物膜, TTC, 脱氢酶活性, 标准曲线

Abstract: The method of 3,5,5-triphenyltetrazolium (TTC) determining biofilms activity was improved,and the problem that the standard curve of determination on TTC-dehydrogenase activity can’t be made conveniently and stably was resolved.The factors which influence the determination were studied.The results showed that sodium sulfide as reducer,toluene as TF organic extractant,Tris-HCl as buffer solution,pH 8.6 and 38 ℃ were the best condition on the developed method,and the optimum reaction time to cultivate biofilms is 6 h.

Key words: biofilms, TTC, dehydrogenase activity, standard curve

李今, 吴振斌, 贺锋. 生物膜活性测定中TTC-脱氢酶活性测定法的改进[J]. journal6, 2005, 26(1): 37-39.

LI Jin, WU Zhen-Bin, HE Feng. Improvement of Determination on TTC-Dehydrogenase Activity in Biofilms[J]. journal6, 2005, 26(1): 37-39.