鳞柄白毒鹅膏菌中α-amanitin的分离与鉴定

journal6 ›› 2004, Vol. 25 ›› Issue (4): 39-43.

鳞柄白毒鹅膏菌中α-amanitin的分离与鉴定

(四川大学生命科学学院生物资源与生态环境教育部重点实验室，四川成都610064)

出版日期:2004-12-15 发布日期:2012-09-22
作者简介:刘军（1971-），男，四川省渠县人，高级工程师，四川大学生命科学学院博士研究生，主要从事生物资源利用与开发研究.
基金资助:
国家十五科技攻关项目(2001BA606A1)

Study on the Separation and Distribution ofα-amanitin in Amanita virosa

(1.College of Life Science,Sichuan University,Chengdu 610064,China;2.West China School of Clinical Medicine,Sichuan University,Chengdu 610041,China)

Online:2004-12-15 Published:2012-09-22

摘要/Abstract

摘要：利用反相高效液相色谱和紫外吸收光谱技术，以乙腈-0.02 mol/L醋酸铵为流动相的梯度洗脱模式，对采集自四川省阿坝州三江自然保护区的鳞柄白毒鹅膏菌子实体中α-鹅膏毒肽含量的分布情况进行研究.研究发现，α-鹅膏毒肽水溶液稳定性很好，其定量分析的线性回归方程为Y=5 159x+1 097.4，鳞柄白毒鹅膏菌子实体中α-鹅膏毒肽的含量为2 838.8 μg/g干子实体.α-鹅膏毒肽在子实体中的分布具有组织特异性，在菌裙中含量最高，约占α-鹅膏毒肽总量的51.09%，且在单位组分中，α-鹅膏毒肽的量也最高约至菌裙组干重的0.29%.

关键词： 鳞柄白毒鹅膏菌；&alpha, -amanitin；反相HPLC

Abstract: The compound α-amanitin from Amanita virosa was separated and determined by reversed-phase high performance liquid chromatography and UV-spectrum,by using 0.02　mol/L aqueous ammonium acetate-acetonitrile as mobile phase with gradient elution mode.The concentration and distribution of α-amanitin in A.virosa carpophores were studied,which were collected in SanJiang nature reserve of ABa zhou in SiChuan province.The results showed that α-amanitin aqueous solution was stable and the linear equation of α-amanitin quantitative analysis was Y=5　159x+1　097.4.The content of α-amanitin reached about 2　838.8　μg per gram dry fruit bodies and the distribution of α-amanitin was tissue dependent.The content of α-amanitin in gills was the highest,which was about 51.09% of total toxin per gram dry carpophores,and the concentration,the 0.29% of dry gills,was also the highest among the different tissues.

Key words: Amanita virosa, &alpha, -amanitin；RP-HPLC

刘军, 张杰, 曹玫, 李达旭, 杨志荣, 赵建. 鳞柄白毒鹅膏菌中α-amanitin的分离与鉴定[J]. journal6, 2004, 25(4): 39-43.

LIU Jun, ZHANG Jie, CAO Mei, LI Da-Xu, YANG Zhi-Rong, ZHAO Jian. Study on the Separation and Distribution ofα-amanitin in Amanita virosa[J]. journal6, 2004, 25(4): 39-43.

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