不同生态型植物对重金属的积累及耐性研究进展

摘要/Abstract

摘要：植物修复是目前治理土壤重金属污染最重要的方法之一，选择合适的植物是植物修复的关键.分类学上种的概念在生态恢复应用上是不充分的，必须从生态型水平上去探讨.不同生态型的同种植物在积累和忍耐重金属方面通常有很大的区别.它们对外源金属的浓度、离子供应形态及金属离子间的相互作用表现出不同的响应特征，富集能力上的差别主要源于不同生态型在金属转运体、解毒机理和遗传特性的不同；金属耐性上的差别主要是由于遗传机制、酶系统、对重金属的避性及区域化不同造成的，植物螯合肽(PC)不是耐性差异的主要因素.不同生态型植物是研究超富集、金属耐性和金属积累机理的理想材料.

关键词： 生态型, 植物修复, 耐性生态型, 敏感生态型

Abstract: Phytoremediation is now regarded as one of the most important techniques to clean soil of heavy metal contamination.It is a key for phytoremediation to select proper plants.Concept of species in taxonomy is not enough in identifying plants for ecological restoration.Rather,consideration must be taken at the level of ecotype.Plants of different ecotype often have large difference in accumulating and tolerating heavy metal,although they are classified as the same species in taxonomy.They could respond differently to variation of concentration,ion form,and interaction of heavy metals.Difference in accumulating heavy metals is mainly attributed to the difference of heavy metal transporter,detoxication and genetics.Difference in enduring heavy metals is due to the difference in genetics,enzyme system and sequestration in cell of different ecotypic plants,while phytochelatins (PC) is not a key factor.Plants of different ecotype are ideal materials in study of hyperaccumulation,toleration and detoxication of heavy metals.This paper introduces the concept,origination and classification of ecotype.It also reviews research progress in heavy metal accumulation and toleration of different ecotypic plants.

Key words: ecotype, phytoremediation, tolerant ecotype, sensitive ecotype

彭少麟, 杜卫兵, 李志安. 不同生态型植物对重金属的积累及耐性研究进展[J]. journal6, 2004, 25(4): 19-26.

PENG Shao-Lin, DU Wei-Bing, LI Zhi-An. A Review of Heavy Metal Accumulation and Tolerance by Plants of Different Ecotype[J]. journal6, 2004, 25(4): 19-26.

参考文献

[1] EHRLICH P R.The Scale of the Human Enterprise[A].SAUNDERS D A,HOBBS R J，EHRLICH P R.In Nature Conservation 3:Reconstruction of Fragmented Ecosystems-Global and Regional Perspectives[C].Surrey Beatty & Sons,Chipping Norton,New South Wales,1993.3-8.

[2] DAILY G C.Restoring Value to the World’s Degraded Lands[J].Science,1995，269:350-354.

[3] SALT D E.Phytoremediation:A Novel Strategy for Removal of Toxic Metals From the Environment Using Plant[J].Bio.Technoligy，1995，13:468-478.

[4] 郑喜坤,鲁安怀,高翔,等.土壤中重金属污染现状与防治方法[J].土壤与环境，2002，11(1):79-84.

[5] 周泽义.中国蔬菜重金属污染及控制[J].资源生态环境网络研究动态，1999，10(3):21-27.

[6] LEF?-BVRA C P.VERNET.Microevolutionary Processes on Contaminatede Deposits[A].SHAW A J.Heavy Metal Tolerance in Plants:Evolutionary Aspects[C].Boca Raton,Florida:CRC Press,Inc.，1990.285-300.

[7] TURESSON G.The Genotypic Response of the Plant Species to the Habitat[J].Hereditas，1922，3:211-350.

[8] ANTONOVICS J,BRADSHAW A D.TUENER R G.Heavy Metal Tolerance in Plants[J].Advance in Ecological Research,1971，7:1-85.

[9] PIETA L F.Evolutionary Response of Plants to Anthropogenic Pollutants[J].Trends in Ecology and Evolution,1988，3:233-236.

[10] 杨万勤,王开运,宋光煜.土壤生态型及其应用[J].中国生态农业学报，2002，10(1):38-40.

[11] 杨肖娥,龙新宪,倪吾钟,等.古老铅锌矿山生态型东南景天对锌耐性及超积累特性的研究[J].植物生态学报,2001，25(6):665-672.

[12] MEERTS P,DUCHENE PH,GRUBER W，et al.Metal Accumulation and Competitive Ability in Metallicolous and Non-Metallicolous Thlaspi Caerulescens Fed With Different Zn Salts[J].Plant and soil,2003,249:1-8.

[13] BAKER A J M.Metal Tolerance[J].New Phytologist，1987，106:93-111.

[14] REEVES R D,BAKER AJM.Studies on Metal Uptake by Plants From Serpentine and Non-Serpentine Populations of Thlaspi Goesingense Halacsy (Cruciferae)[J].New Phytologist，1984，98:191-204.

[15] CHANEY R L,MALIK M,LI Y M,et al.Phytoremediation of Soil Metals[J].Curr Opin Biotechnol,1997,8:279-84.

[16] LOMBI E,ZHAO F J,DUNHAM S J,et al.Cadmium Accumulation in Populations of Thlaspi Caerulescens and Thlaspi goesingense[J].New Phytol，2000，145:11-20.

[17] BAKER A J M,WALKER P L.Ecophysiology of Metal Uptake by Tolerant Plants[A].SHAW A J.Heavy Metal Tolerance in Plants:Evolutionary Aspects[C].Boca Raton：FL,CRC Press,1990.155-177.

[18] 黄铭洪，等.环境污染与生态恢复[M].北京.科学出版社，2003.

[19] OSTERA S,EKVALL A H,GREGER L M.Sensitivity to and Accumulation of Cd in Betula Pendula,Picea Abies and Pinus Sylvestris Seedlings From Different Regions in Sweden[J].Canadian Journals of Botany.2000，78,1-11.

[20] LOMBI E,ZHAO F J,MCGRATH S P,et al.Physiological Evidence for a High Affinity Cadmium Transporter in a Thlaspi caerulescens Ecotype[J].New Phytol ，2001，149:53-60.

[21] LANDBERG T，GREGER M.Differences in Uptake and Tolerance to Heavy Metals in Salix From Unpolluted and Polluted Areas[J].Applied Geochemistry.1996，11:175-180.

[22] QURESHI J A,THURMAN D A,HARDWICK K,et al.Uptake and Accumulation of Zinc,Lead and Copper in Zinc and Lead Tolerant Anthoxanthum Odoratum L[J].New Phytologist，1985，100：429-434.

[23] LOLKEMA P C,DONKER M H,SCHOUTEN A J,et al.The Possible Role of Metallothioneins in Copper Tolerance of Silene Cucubalus[J].Planta，1984，162：174-179.

[24] WU L,LIN S L.Copper Tolerance and Copper Uptake of Lotus Purshianus (Benth.)Clem.& Clem.and Its Symbiotic Rhizobium Loti Derived From a Copper Mine Waste Population[J].New Phytologist，1990，116：531-539.

[25] BRADLEY R,BURT A J,READ D J.The Biology of Mycorrhiza in the Ericaceae VIII：The Role of Mycorrhizal Infection in Heavy Metal Resistance[J].New Phytologist，1982，91：197-209.

[26] EKVALL L，GREGER M.Effects of Environmental Biomass-Producing Factors on Cd Uptake in Two Swedish Ecotypes of Pinus sylvestris[J].Environmental Pollution，2002，121：401-411.

[27] LOMBI E,TEARALL K L,HOWARTH J R,et al.Influence of Iron Status on Cadmium and Zinc Uptake by Different Ecotypes of the Hyperaccumulator Thlaspi Caerulescens[J].Plant Physiology,2002,128:1 359-1 367.

[28] ZHAO Fang-jie,REBECCA E,HAMON,et al.McGrath Characteristics of Cadmium Uptake in Two Contrasting Ecotypes of the Hyperaccumulator Thlaspi caerulescens[J].J.Exp.Bot.,2002,53:535-543.

[29] ASSUNCAO A G L,DE COSTA MARTINS P,DE FOLTER S,et al.Elevated Expression of Metal Transporter Genes in Three Accessions of the Metal Hyperaccumulatior Thlaspi Caerulescens[J].Plant Cell Environ,2001,24:217-226.

[30] RENGEL Z.Ecotype of Holcus Lanatus Tolerant to Zinc Toxicity Also Tolerate Zinc Deficiency[J].Annals of botany，2000，86:1 119-1 126.

[31] HARMENS H,DEN HARTOG P R,TEN BOOKEM W M,et al.Increased Zinc Tolerance in Silene Vulgaris (Moench) Garcke is Not Due to Increased Production of Phytochelatins[J].Plant Physiology，1993，103(4):1 305-1 309.

[32] VERKLEIJ JAC,KOEVOETS PLM,BLAKE-KALFF MMA,et al.Evidence for an Important Role of the Tonoplast in the Mechanism of Naturally Selected Zn Tolerance in Silene Vulgaris[J].J.Plant Physiol，1998，153:188-191.

[33] SHEN Z G,ZHAO F J,MCGRATH S P.Uptake and Transport of Zinc in the Hyperaccumulator Thlaspi Caerulescens and the Non-Hyperccumulator Thlaspi Ochroleucum[J].Plant,Cell and Environment，1997，20:898-906.

[34] WHITING S N,LEAKE J R,MCGRATH S P,et al.Positive Response to Zn and Cd by Roots of the Zn and Cd Hyperaccmulator Thlaspi Caerulescens[J].New Phytol,2000,145:199-210.

[35] ANTONOVICS J.Metal Tolerance in Plants:Perfecting an Evolutionary Paradigm[A].Proceedings of the First International Conference on Heavy Metals in the Enironment[C].Toronto,Canada,1975，2:169－186.

[36] MACNAIR M R.The Genetics of Metal Tolerance in Vascular Plants.Tansley Review No[J].49.New Phytologist，1993，124：541-559.

[37] MCNAUGHTON S J， FOLSOM T C,LEE T,et al.Heavy Metals Tolerance in Typha Latifolia Without the Evolution of Tolerant Races[J].Ecology，1974，55：1 163-1 165.

[38] TAYLOR G J,CROWDER A A.Copper and Nickel Tolerance in Typha Latifolia Clones From Contaminated and Uncontaminated Environments[J].Canadian Journal of Botany，1984，62:1 304-1 308.

[39] BARBARA PAWLIK-SKOWROńSKA.Resistance,Accumulation and Allocation of Zinc in Two Ecotypes of the Green Alga Stigeoclonium tenue Kütz[A].Coming From Habitats of Different Heavy Metal Concentrations[C].Aquatic Botany，2003，75:189-198.

[40] SHU W S，YE Z H.Lead Zinc and Copper Accumulation and Tolerance in Populations of Paspalum distichum and Cynodon Dactylon[J].Environmental pollution，2002，120:445-453.

[41] SCHAT H,KALFF M M A.Are Phytochelatins Involved in Differential Metal Tolerance or do the Merely Reflect Metal-Imposed Strain?[J].Plant Physiol，1992，99:1 475-1 480.

[42] CRISTINA GONNELLI.Francesca Galardi and Roberto Gabbrielli：Nickel and Copper Tolerance and Toxicity in Three Tuscan Populations of Silene Paradoxa[J].Physiologia Plantaru,2002，113:(4) 507-514.

[43] INGROUILLE M J，SMIRNOFF N.Thlaspi Caerulescens J.and C.Presl (T.alpestre L.) in Britain[J].New Phytol，1996，102:219-233.

[44] GIBSON D J，RISSER P G.Evidence for the Absence of Ecotypic Development in Andropogon Virginicus L.on Metalliferous Mine Wastes[J].New phytol，1982， 92:589-599.

[45] SMITH S E，MACNAIR M R.Hypostatic Modifiers Cause Variation in Degree of Copper Tolerance in Mimulus guttatus[J].Heredity，1998，80:760-768.

[46] MEHARG A A，MACNAIR M R.Suppression of the Phosphate Uptake System:A Mechanism of Arsenate Tolerance in Holcus lanatus[J].Journal of Experimental Botany,1992，43:519-524.

[47] RAUSER W E.Structure and Function of Metal Chelators Produced by Plants:The Case for Organic Acids,Amino Acids,Phytin and Metallothioneins[J].Cell Biochem Biophys，1999，31:19-48.

[48] MURPHY A,TAIZ L.Comparison of Metallothionein Gene Expression and Nonprotein Thiols in 10 Arabidopsis Ecotypes[J].Plant Physiol，1995，109:945-954.

[49] VAN HOOF N A L M,HASSINEN V H,HAKVOORT H,et al.Enhanced Copper Tolerance in Silene Vulgaris (Moench) Garcke Populations From Copper Mines is Associated With Increased Transcript Levels and a Tandem Repeat of a 2b-Type Metallothionein Gene[J].Plant Physiology，2001，126:1 519-1 526.

[50] SCHAT H,VOOIJS R,KUIPER E.Identical Major Gene Loci for Heavy Metal Tolerances That Have Independently Evolved in Different Local Populations and Subspecies of Silene Vulgaris[J].Evolution，1996，50:1 888-1 895.

[51] BERT V,MACNAIR M R,DELAGUERIE P，et al.Zinc Tolerance and Accumulation in Metallicolous and Nonmetallicolous Populations of Arabidopsis Halleri (Brassicaceae) [J].New Phytologist,2000,146:225-233.

[52] SCHAT H,VOOIJS R.Multiple Tolerance and Co-Tolerance to Heavy Metals in Silene vulgaris:A Co-Segregation Analysis[J].New Phytol,1997，136:489-496.

[53] 叶志鸿.锌污染对植物的毒害及植物的忍耐性[J].生态学杂志,1992，11(5):42-45.

[54] 杨居荣,黄翌.植物对重金属的耐性机理[J].生态学杂志,1994，13(6):20-26.

[55] MATHYS W.Vergleichende Under Suchugen der Zinkaufnabme Von Die Sistenten und Senditive Population[J].Von Agrostis Tenuis Sibth.Flora，1973，162:492-499.

[56] DE VOS CHR,VONK M J,VOOIJS R,et al.Glutathione Depletion Due to Copper-Induced Phytochelatin Synthesis Causes Oxidative Stress in Silene Cucubalus[J].Plant Physiol，1992，98:853-858.

[57] LLUGANY M,LOMBINI A,POSCHENRIEDER C,et al.Different Mechanisms Account for Enhanced Copper Resistance in Silene Armeria Ecotypes From Mine Spoil and Serpentine Sites[J].Plant Soil，2003，251:55-63.

[58] GRILL E,WINNACKER E L,ZENK M H.Phytochelatins,a Class of Heavy-Metal-Binding Peptides From Plants are Functionally Analogous to Metallothioneins[J].Proc Natl Acad Sci USA.，1987，84:439-443.

[59] DE KNECHT J A,KOEVOETS P L M,VERKLEIJ J A C,et al.Evidence Against a Role for Phytochelatins in Naturally Selected Increased Cd Tolerance in Silene Vulgaris (Moench.) Garcke[J].New Phytol，1992，122:681-688.

[60] DE KNECHT J A,DILLEN M,KOEVEOETS P L M,et al.Phytochelatins in Cadmium-Sensitive and Cadmium-Tolerant Silene Vulgaris[J].Plant Physiol，1994，104:255-261.

[61] BRUNE A,URBACHW DIETZ K-J.Compartmentation and Transpor of Zinc in Barley Primary Leaves as Basic Mechanisms Involved in Zinc Tolerance[J].Plant,Cell and Environment，1994，17:153-162.

[62] ERNST W H O,VERKLEIJ J A C,SCHAT H.Metal Tolerance in Plants Acta Botanica Neerlandica，1992，41:229-248.

[63] GRILL E,L?;FFLER S,WINNACKER E L,et al.Phytochelatins,the Heavy Metal Binding Peptides of Plants,are Synthesized From Glutathione by a Specific G-Glutamylcysteinedipeptyl Transpeptidase (Phytochelatin Synthase)[J].Proc Natl Acad Sci.USA，1989，86:6 838-6 842.

[64] ERNST W H O.Zur Physiologie Der Schwermetallpflanzen:Subzellulre Speicherungs-Ortedes Zinks[J].Ber Dtsch Bot Ges，1969，82:161-164.

[65] MATHYS W.Enzymes of Heavy-Metal-Resistant and Nonresistant Populations of Silene Cucubalus and Their Interaction With Some Heavy Metals in Vitro and in Vivo[J].Physiol Plant，1975，33:161-165.

[66] TURESSON G.The Species and the Variety as Ecological Units[J].Hereditas，1922，3:100-113.

[67] CLAUSEN J,KECK D D,HIESEY W M.Experimental Studies on the Nature of Species,III:Environmental Responses of Climatic Races of Archillea[J].Carnegie Institution of Washington Publication，1948，581:1-129.

[68] BRADSHAW A D,CHADWICK J..The Restoration of Land[J].Blackwell Scientific Publications,Oxford，1980.

[69] WONG M H,LAU W M,LI S W,et al.Root Growth of two Grass Species on Iron ore Tailings at Elevated Levels of Manganese,Iron and Copper[J].Environmental Research，1983，30：26-33.

[70] JOWETT D.Population Studies on Lead Tolerant Agrositis Tenius[J].Evolution，1964，18：70-81.