journal6 ›› 2002, Vol. 23 ›› Issue (3): 56-58.

• 重点学科 • 上一篇    下一篇

银杏根中黄酮类化合物的提取及其含量测定

  

  1. (1.吉首大学资源与环境科学学院,湖南 吉首 416000;2.吉首大学实验中心,湖南 吉首 416000)
  • 出版日期:2002-09-15 发布日期:2013-01-05
  • 作者简介:李洪雄(1976-)男,湖南省新化县人,吉首大学资源与环境学学院助教,主要从事分析化学研究.
  • 基金资助:

    湖南省教委自然科学基金资助项目(00C193)

Extraction and Determination of Content of Flavonoids in the Root of Ginkgoes

  1. (1.College of Resources and Environment,Jishou University,Jishou 416000,Hunan china;2.The Centre of Analysis and Measurement,Jishou University,Jishou 416000,Hunan China)
  • Online:2002-09-15 Published:2013-01-05

摘要:采用浸泡与回流的方法从银杏根中提取黄酮,用721型分光光度计测得总黄酮含量为6.89%.高效液相色谱法检测银杏根中槲皮素百分率为1.24%,是黄酮类化合物中含量较高的成分.紫外-可见分光光度法确定最佳检测波长为370 nm.

关键词: 分光光度计, 高效液相色谱, 黄酮类化合物, 槲皮素, 银杏根

Abstract: Flavonoids was extracted from the foot of the ginkgoes by the method of soaking and backwater.The content of flavonoids determined with a spectrophoto meter of model 721 was 6.89%.In the root of ginkgoes,the content of quercetin accounted 1.24%.It was determined by high performance liquid chromatography.The optimal detection wave length settled by ultiaviolet and visible spectrophoto metry.

Key words: spectrophoto meter, high performance liquid chromatograph flavonoids, quercetin, the root of the ginkgoes

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