非洲菊的组织培养

journal6 ›› 2001, Vol. 22 ›› Issue (1): 4-6.

非洲菊的组织培养

( 吉首大学生命科学与化学学院,湖南吉首 416000

出版日期:2001-03-15 发布日期:2013-01-05
作者简介:黄衡宇( 1970- ) ,男, 江苏省无锡市人,生物学硕士,吉首大学生命科学与化学学院讲师,主要从事植物胚胎学研究.

Tissue Culture of Gerbera jamesonii Bolus

( The Biology Department of Jishou University, Jishou 416000,Hunan China)

Online:2001-03-15 Published:2013-01-05

摘要/Abstract

摘要：针对非洲菊自然繁殖率低下的情况,探讨了利用组织培养方法解决生产中繁殖的问题. 结果显示: 在茎段、茎尖和叶柄3种外植体中,茎尖的出愈率最高, 是理想的快速繁殖材料,较适宜的诱导愈伤组织的培养基为MS+ BA 1. 0 mg•L^-1+IBA 0. 0 5 mg•L^-1+庶糖3. 0%,诱导不定芽的培养基为MS+ BA 2. 0 mg•L^-1+IAA 0. 5 mg•L^-1+ 庶糖3. 0%或MS+ Kt 3. 0+ IAA0. 05 mg•L^-1+ 庶糖3. 0%, 而根的诱导则是在 MS+ NAA 0. 3 mg•L^-1+ IBA 0. 3 mg•L^-1+庶糖3. 0%的培养基上进行.

关键词： 非洲菊, 组织培养, 愈伤组织, 不定芽

Abstract: Because the fruiting rate and germinat ive percentage of Gerbe rajame sonii Bolus are lower, tissue culture was explored to solve the propagating problem in the product ion. The results show that the shoot-tip is the best mate rial in speeding propagation among the three explants ( shoot- tip, stem and petiole) as it has the bigges tinductivity,the best growth and it is easy to be inducted adventitious buds.The suitable phytohormone compositions toninduct callus, adventitious buds and roots are respectively: MS+ BA 1. 0 mg•L^-1+ IBA 0. 05mg•L^-1+sugar 3.0%, Ms+BA2. 0 mg•L^-1+ IAA 0. 5 mg•L^-1+ sugar 3. 0% or Ms+ Kt 3. 0 mg•L^-1+ IAA 0. 05 mg•L^-1+ sugar 3. 0% , and Ms+ NAA 0. 3 mg•L^-1+ IBA 0. 3 mg•L^-1+ sugar 3. 0%.

Key words: Gerbera jamesonii Bolus, tissue culture, callus, adventitious bud

黄衡宇, 李鹂, 杨胜辉. 非洲菊的组织培养[J]. journal6, 2001, 22(1): 4-6.

HUANG Heng-Yu, LI Li, YANG Sheng-Hui. Tissue Culture of Gerbera jamesonii Bolus[J]. journal6, 2001, 22(1): 4-6.

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