具有ACC脱氨酶活性的绞股蓝根际细菌的分离鉴定及其促生作用

doi:10.3969/j.issn.1007-2985.2014.05.011

journal6 ›› 2014, Vol. 35 ›› Issue (5): 43-50.DOI: 10.3969/j.issn.1007-2985.2014.05.011

具有ACC脱氨酶活性的绞股蓝根际细菌的分离鉴定及其促生作用

SONG Jin-Qiu, WU Lu-Mu-Han-·Na-Zi-er-Bie-Ke, ZHANG Ti, En-Te-Ma-Ke-·Bu-La-Ti-Bai

（吉首大学生物资源与环境科学学院，湖南吉首 416000）

出版日期:2014-09-25 发布日期:2014-10-30
通讯作者: 恩特马克?布拉提白（1961—），男，新疆人，吉首大学生物资源与环境科学学院教授，博士，主要从事动物病原微生物学研究；E-mail etmkb@jsu.edu.cn.
作者简介:宋金秋（1982—），女，湖南吉首人，吉首大学生物资源与环境科学学院硕士生，主要从事微生物生态学研究
基金资助:
生态旅游湖南省重点实验室开放基金资助项目（JDSTLY201202）；国家自然科学基金资助项目（31260039）

Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of Gynostemma Pentaphyllum Makino

宋金秋, 吾鲁木汗•那孜尔别克, 张缇, 恩特马克•布拉提白

(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)

Online:2014-09-25 Published:2014-10-30

摘要/Abstract

摘要：为了筛选含ACC脱氨酶的根际促生细菌，以1-氨基环丙烷-1-羧酸（ACC）为唯一氮源，从绞股蓝根际土壤中富集和分离ACC脱氨酶阳性细菌，利用α-丁酮酸测定法和CAS平板法分别检测其ACC脱氨酶活性和产嗜铁素能力，利用菌液培养接种法检测细菌的促生作用，最后通过生理生化试验和16S rRNA基因序列分析对所筛选的菌株进行鉴定.经富集筛选法从绞股蓝根际土壤中分离出9株ACC脱氨酶阳性细菌，它们均具有不同程度的ACC脱氨酶活性，其中3株细菌能产生嗜铁素.促生试验结果表明，7株ACC脱氨酶活性细菌对水稻幼苗根系的生长发育表现出不同程度的促生作用，其中菌株JDG127的促生作用最明显，与对照组相比，水稻幼苗的根长和根鲜重增长了约1.6倍.鉴定结果表明，9株细菌中1株属于单胞菌属（Stenotrophomonas），1株属于类芽胞杆菌属（Paenibacillus），1株属于芽孢杆菌属（Bacillus），2株属于肠杆菌属（Enterobacter），4株属于不动杆菌属（Acinetobacter），与ACC脱氨酶相比，铁载体对根际细菌的促生作用更明显.

关键词： 绞股蓝, 根际细菌, ACC脱氨酶, 促生能力

Abstract: A total of 9 bacterial strains were isolated from rhizosphere of Gynostemma pentaphyllum through enrichment on 1-aminocyclopropane-1-carboxylate (ACC) as a sole nitrogen source.ACC deaminase assay showed that all isolates differed in their potential for ACC deaminse activity,among which 3 strains were positive for siderosphere by chrome azurol S (CAS) assay.Inoculation of rice seedlings with 7 ACC deaminase-containing strains had significant effect on root elongation and root fresh weight,among which the strain JDG127 caused 1.6-fold increase in root length and root fresh weight over uninoculated control.Based on the physiological and biochemical characteristics in combination with 16S rRNA gene sequences analysis,the 9 ACC deaminase- containing strains were identified as Stenotrophomonas (1 strain),Paenibacillus (1 strain),Bacillus (1 strain),Enterobacter (2 strains) and Acinetobacter (4 strains).The results indicated that the siderosphere producing ability of rhizobacteria may be correlated with their plant growth-promoting ability when compared to their ACC-deaminase activity.

Key words: Gynostemma pentaphyllum, rhizobacteria, ACC deaminase, growth-promoting ability

宋金秋, 吾鲁木汗?那孜尔别克, 张缇, 恩特马克?布拉提白. 具有ACC脱氨酶活性的绞股蓝根际细菌的分离鉴定及其促生作用[J]. journal6, 2014, 35(5): 43-50.

SONG Jin-Qiu, WU Lu-Mu-Han-·Na-Zi-er-Bie-Ke, ZHANG Ti, En-Te-Ma-Ke-·Bu-La-Ti-Bai . Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of Gynostemma Pentaphyllum Makino[J]. journal6, 2014, 35(5): 43-50.

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具有ACC脱氨酶活性的绞股蓝根际细菌的分离鉴定及其促生作用

Isolation and Plant Growth-Promoting Ability of ACC Deaminase-Containing Bacteria from Rhizosphere of Gynostemma Pentaphyllum Makino

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