基于聚邻苯二胺复合膜电位型免疫传感器的制备

doi:10.3969/j.issn.1007-2985.2013.04.015

journal6 ›› 2013, Vol. 34 ›› Issue (4): 67-71.DOI: 10.3969/j.issn.1007-2985.2013.04.015

基于聚邻苯二胺复合膜电位型免疫传感器的制备

(辽宁师范大学化学化工学院功能材料化学研究所,辽宁大连 116029)

出版日期:2013-07-25 发布日期:2013-07-18
作者简介:冯春梁(1956-),男,辽宁岫岩人,辽宁师范大学化学化工学院教授，主要从事电化学、生物传感器、酶催化动力学研究.
基金资助:
国家自然科学基金资助项目(60572009);辽宁省教育厅高校重点实验室项目(2008S134)

Preparation of Potentiornetric Immunosensor Based on Poly (o-Phenylenediamine) Film

(School of Chemistry and Chemical Engineering,Institute of Chemistry for Functionalized Materials,Liaoning Normal University,Dalian 116029,China)

Online:2013-07-25 Published:2013-07-18

摘要/Abstract

摘要：首先利用循环伏安法制备聚邻苯二胺(POPD)修饰碳糊电极(CPE/POPD)，然后在CPE/POPD上电沉积普鲁士蓝(PB) 和纳米金(GNPs)，制备CPE/POPD/PB-GNPs电极，最后将羊抗小鼠IgG通过金-氨键固定在CPE/POPD/ PB-GNPs上，从而制得一种免疫传感器(CPE/POPD/PB-GNPs/Ab).用循环伏安法和电化学交流阻抗技术对电极的修饰过程进行表征.利用所制备的免疫传感器对溶液中的小鼠IgG进行检测，结果表明，在2.0×10-5 μg/L和1.0×04 μg/L 2种浓度下，免疫传感器均得到了很稳定的电位响应信号，响应时间不超过3 min.所制备的免疫传感器具有灵敏度高、响应速度快、检测范围宽、成本低廉等突出优点.

关键词： 聚邻苯二胺, 纳米金, 普鲁士蓝, 抗体

Abstract: Firstly,o-phenylenediamine(OPD) was electropolymerized on carbon paste electrode（CPE/POPD）by cyclic voltammetry method.Then the gold nanoparticals (GNPs) and prussian blue (PB) were deposited onto the CPE/POPD by electrodeposition technology to fabricate CPE/POPD/PB-GNPs electrode.Finally,the goat anti-mouse IgG (antibody,Ab) were immobilizeded on to the CPE/POPD/PB-GNPs electrode through Au-NH-bond,and thus a potentiometric immunosensor (CPE/POPD/PB-GNPs/Ab) was sucessfully prepared.The modified process of the electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).The immunosensor exhibited fast potentiometric response (<3 min) under the antigen concentration of 2.0×10-5 μg/L and 1.0×104 μg/L when it was used to detect the mouse IgG.The results of experiments showed that the immunosensor based on POPD/GNPs-PB composite film exhibited the advantages of high sensitivity,fast response,wide detection range and low cost.

Key words: poly (o-phenylenediamine), nano gold, prussian blue, antibody

冯春梁, 柏雨辰, 孙越, 杜朋, 王璐. 基于聚邻苯二胺复合膜电位型免疫传感器的制备[J]. journal6, 2013, 34(4): 67-71.

FENG Chun-Liang, BAI Yu-Chen, SUN Yue, DU Peng, WANG Lu. Preparation of Potentiornetric Immunosensor Based on Poly (o-Phenylenediamine) Film[J]. journal6, 2013, 34(4): 67-71.

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[2]	石杰, 范淑敏, 吴静, 康小慧, 尹瑜静, 王瑞勇. 生物法合成纳米金的研究进展[J]. journal6, 2012, 33(3): 71-75.

基于聚邻苯二胺复合膜电位型免疫传感器的制备

Preparation of Potentiornetric Immunosensor Based on Poly (o-Phenylenediamine) Film

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