淹水胁迫下抗氧化酶活性对对萼猕猴桃的耐涝性影响

doi:10.13438/j.cnki.jdzk.2025.06.009

吉首大学学报（自然科学版） ›› 2025, Vol. 46 ›› Issue (6): 67-74.DOI: 10.13438/j.cnki.jdzk.2025.06.009

淹水胁迫下抗氧化酶活性对对萼猕猴桃的耐涝性影响

徐零零，张帆靖，罗庆，徐艳玲，高建有，田晓波，查满荣，刘世彪

（1.吉首大学生命科学学院，湖南吉首 416000；2.张家界学院，湖南张家界 427000；3.中国科学院广西植物研究所，广西桂林 541006；4.永顺县砂坝镇人民政府，湖南永顺 416700）

出版日期:2025-11-25 发布日期:2025-12-17
作者简介:徐零零（2000—），女，江西九江人，吉首大学生命科学学院硕士研究生，主要从事猕猴桃抗涝性研究
基金资助:
国家自然科学基金资助项目（32160084）；湖南省自然科学基金资助项目（2023JJ30488）；湖南省教育厅科学研究项目（23B0502）

Effects of Antioxidant Enzyme Activities on Waterlogging Tolerance of Actinidia valvata Under Waterlogging Stress

XU Lingling,ZHANG Fanjing,LUO Qing,XU Yanling,GAO Jianyou,TIAN Xiaobo,ZHA Manrong,LIU Shibiao

(1.College of Life Sciences,Jishou University,Jishou 416000,Hunan China;2.Zhangjiajie University,Zhangjiajie 427000,Hunan China;3.Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin 541006,Guangxi China;4.People's Government of Shaba Town,Yongshun County,Yongshun 416700,Hunan China)

Online:2025-11-25 Published:2025-12-17

摘要/Abstract

摘要：以对萼猕猴桃“水秀”品种为试材，以中华猕猴桃“红阳”品种为物种对照，检测人工淹水处理6 h（前期）、24 h（中期）、120 h（后期）2种猕猴桃根系的生理指标变化.结果表明，在淹水前期，“水秀”根系的丙二醛（MDA）质量摩尔浓度较高，之后保持较低水平；在淹水前、中期，“水秀”根系的超氧阴离子（O-2）质量摩尔浓度均较低；在淹水前、中期，“水秀”的根系活力较强；在淹水前、中、后期，“水秀”根系的超氧化物歧化酶（SOD）活性均维持在较高水平；随着淹水胁迫时间的延长，“水秀”根系的过氧化氢酶（CAT）活性先升高后降低，过氧化物酶（POD）活性先降低后升高；“水秀”淹水处理组根系3种抗氧化酶活性均始终明显高于对照组，整体也高于“红阳”淹水处理组.“水秀”面对淹水胁迫能迅速响应并启动抗氧化防御系统，及时减轻淹水胁迫引发的氧化损伤，表现出较强的耐涝性；“红阳”的相关生理指标基本上与“水秀”相反，表现出较弱的耐涝性.

关键词： 对萼猕猴桃, 中华猕猴桃, 淹水胁迫, 根系活力, 抗氧化酶活性

Abstract: The root physiological changes of two kiwifruit varieties,Actinidia valvata "Shuixiu" and Actinidia chinensis "Hongyang",were investigated under artificial waterlogging treatments of 6 h (early stage),24 h (middle stage),and 120 h (late stage).The results showed that in the early stage of waterlogging,the malondialdehyde (MDA) molar concentration in the roots of Actinidia valvata "Shuixiu" was relatively high,but then remained at a low level.In the early and middle stages of waterlogging,the molar concentration of superoxide anion (O-2) in the roots of Actinidia valvata "Shuixiu" was relatively low.In the early and middle stages of waterlogging,the root activity of Actinidia valvata "Shuixiu" was relatively strong.In the early,middle,and late stages of waterlogging,the superoxide dismutase (SOD) activity in the roots of Actinidia valvata "Shuixiu" remained at a relatively high level.With the extension of waterlogging stress time,the catalase (CAT) activity in the roots of Actinidia valvata "Shuixiu" first increased and then decreased,while the peroxidase (POD) activity first decreased and then increased.The activities of the three antioxidant enzymes in the roots of the Actinidia valvata "Shuixiu" waterlogging treatment group were always significantly higher than those of the control group,and were also higher than those of the Actinidia chinensis "Hongyang" waterlogging treatment group overall.Actinidia valvata "Shuixiu" can rapidly respond to waterlogging stress and activate the antioxidant defense system in time to alleviate the oxidative damage caused by waterlogging stress,showing strong waterlogging tolerance.The above indicators of Actinidia chinensis "Hongyang" were basically opposite to those of Actinidia valvata "Shuixiu",showing weak waterlogging tolerance.

Key words: Actinidia valvata, Actinidia chinensis, waterlogging stress, root vigor, antioxidant enzyme activity

徐零零, 张帆靖, 罗庆, 徐艳玲, 高建有, 田晓波, 查满荣, 刘世彪. 淹水胁迫下抗氧化酶活性对对萼猕猴桃的耐涝性影响[J]. 吉首大学学报（自然科学版）, 2025, 46(6): 67-74.

XU Lingling, ZHANG Fanjing, LUO Qing, XU Yanling, GAO Jianyou, TIAN Xiaobo, ZHA Manrong, LIU Shibiao. Effects of Antioxidant Enzyme Activities on Waterlogging Tolerance of Actinidia valvata Under Waterlogging Stress[J]. Journal of Jishou University(Natural Sciences Edition), 2025, 46(6): 67-74.

参考文献

[1] CALABRITTO MARIA,MININNI ALBA N,DI BIASE ROBERTO,et al.Physiological and Image-Based Phenotyping Assessment of Waterlogging Responses of Three Kiwifruit Rootstocks and Grafting Combinations[J].Frontiers in Plant Science,2025,16:1499432.DOI:10.3389/fpls.2025.1499432.
[2] XING Mengyun,HUANG Kangkang,ZHANG Chen,et al.Transcriptome Analysis Reveals the Molecular Mechanism and Responsive Genes of Waterlogging Stress in Actinidia deliciosa Planch Kiwifruit Plants[J].International Journal of Molecular Sciences,2023,24(21):15887.DOI:10.3390/ijms242115887.
[3] 米银法.猕猴桃实生苗对根际低氧胁迫的抗性评价及抗性生理机制研究[D].咸阳:西北农林科技大学,2009.
[4] 镇熙,林诗艳,王娜灵,等.超声对猕猴桃溃疡病的抑制作用[J].吉首大学学报 (自然科学版),2024,45(3):29-34.
[5] 徐零零,徐艳玲,高建有,等.淹水胁迫对对萼猕猴桃光合特性的影响[EB/OL].(2025-06-20)[2025-06-28].https:∥doi.rog/10.13938/j.issn.1007-1431.20240570.
[6] 郭晓成,杨莉,段眉会,等.再说猕猴桃新型砧木水杨桃[J].西北园艺:果树,2019(6):4-6.
[7] 刘扬,谢善鹏,卢鑫,等.不同砧木对红阳猕猴桃生长及果实品质的影响[J].落叶果树,2020,52(6):11-14.
[8] 高磊,罗轩,汪志,等.利用对萼猕猴桃砧木建园与栽培管理技术[J].中国果树,2024,2024(11):108-113.
[9] 陈玉明,史梦琪,张琮,等.耐淹砧木对猕猴桃枝叶生长及淹水胁迫的生理影响[J].湖北农业科学,2018,57(8):77-80.
[10] BAI Danfeng,LI Zhi,HU Chungen,et al.Transcriptome-Wide Identification and Expression Analysis of ERF Family Genes in Actinidia valvata During Waterlogging Stress[J].Scientia Horticulturae,2021,281(1):109994.DOI:10.1016/j.scienta.2021.109994.
[11] LI Zhi,BAI Danfeng,ZHONG Yunpeng,et al.Full-Length Transcriptome and RNA-Seq Analyses Reveal the Mechanisms Underlying Waterlogging Tolerance in Kiwifruit (Actinidia valvata)[J].International Journal of Molecular Sciences,2022,23(6):3237.DOI:10.3390/ijms23063237.
[12] CHEN Yue,SU Wenyue,REN Chunjiang,et al.Restricted Responses of AcMYB68 and AcERF74/75 Enhanced Waterlogging Tolerance in Kiwifruit[J].Plant Journal,2024,119(2):1059-1072.
[13] 罗庆.基于糖代谢途径的对萼猕猴桃耐涝机制研究[D].吉首:吉首大学,2023.
[14] GAO Minxia,GAI Chaoyue,LI Xinyu,et al.Waterlogging Tolerance of Actinidia valvata Dunn is Associated with High Activities of Pyruvate Decarboxylase,Alcohol Dehydrogenase and Antioxidant Enzymes[J].Plants (Basel),2023,12(15):2872.DOI:10.3390/plants12152872.
[15] ZHANG Meijuan,LIU Cuixia,WANG Faming,et al.Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and an Expression Analysis of Their Responses to Waterlogging Stress[J].Horticulturae,2024,10(4):310.DOI:10.3390/horticulturae10040310.
[16] ZHANG Meijuan,YE Kaiyu,GAO Jianyou,et al.Identification and Functional Analysis of AvLACS Genes Unveils Their Role in Lipid Homeostasis and Waterlogging Tolerance in Kiwifruit (Actinidia valvata Dunn)[J].Frontiers in Plant Science,2025,16:1580003.DOI:10.3389/fpls.2025.1580003.
[17] 杨江平,向小奇,唐玲玲,等.野生猕猴桃LD-1的耐涝性研究[J].湖南农业科学,2014(23):9-10;14.
[18] 魏汐悦.两种猕猴桃无性系砧木对淹水胁迫的生理响应研究[D].武汉:华中农业大学,2024.
[19] NARAYAN BHUSAL,HYUN SEOK KIM,HAN SU-GON,et al.Photosynthetic Traits and Plant-Water Relations of Two Apple Cultivars Grown as Bi-Leader Trees Under Long-Term Waterlogging Conditions[J].Environmental and Experimental Botany,2020,176:104111.DOI:10.1016/j.envexpbot.2020.104111.
[20] TIAN Lixin,ZHANG Yuchuan,CHEN Pengliang,et al.How Does the Waterlogging Regime Affect Crop Yield? A Global Meta-Analysis[J].Frontiers in Plant Science,2021,12:634898.DOI:10.3389/fpls.2021.634898.
[21] 聂功平,陈敏敏,杨柳燕,等.植物响应淹水胁迫的研究进展[J].中国农学通报,2021,37(18):57-64.
[22] 陈月.‘东红’和‘对萼’猕猴桃耐涝性比较及差异基础分析[D].杭州:浙江大学,2023.
[23] JRGEN KREUZWIESER,HEINZ RENNENBERG.Molecular and Physiological Responses of Trees to Waterlogging Stress[J].Plant Cell & Environment,2014,37(10):2245-2259.
[24] NARAYAN BHUSAL,IN HEE PARK,SANGHAK JEONG,et al.Photosynthetic Traits and Plant Hydraulic Dynamics of Gamhong Apple Cultivar Under Drought and Waterlogging Conditions and Subsequent Recovery from Stress Conditions[J].SSRN Electronic Journal,2023,321:112276.DOI:10.1016/j.scienta.2023.112276.
[25] PAN Taowen,ZHANG Jian,HE Lanmengqi,et al.Silicon Enhances Plant Resistance of Rice Against Submergence Stress[J].Plants (Basel),2021,10(4):767.DOI:10.3390/plants10040767.

淹水胁迫下抗氧化酶活性对对萼猕猴桃的耐涝性影响

Effects of Antioxidant Enzyme Activities on Waterlogging Tolerance of Actinidia valvata Under Waterlogging Stress

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价