超声对猕猴桃溃疡病的抑制作用

doi:10.13438/j.cnki.jdzk.2024.03.005

吉首大学学报（自然科学版） ›› 2024, Vol. 45 ›› Issue (3): 29-34.DOI: 10.13438/j.cnki.jdzk.2024.03.005

超声对猕猴桃溃疡病的抑制作用

镇熙，林诗艳，王娜灵，张棕豪，黎骏杰，谭泽成，刘世彪，查满荣，王琰

(吉首大学生物资源与环境科学学院，湖南吉首 416000)

出版日期:2024-05-25 发布日期:2024-06-19
通讯作者: 王琰（1990—），女，湖南古丈人，吉首大学生物资源与环境科学学院讲师，博士，主要从事作物学研究.E-mail:wy90408@163.com.
基金资助:
国家自然科学基金资助项目（32160084）；湖南省自然科学基金资助项目（2023JJ30488）；湖南省大学生创新创业训练计划项目（S202210531044）

Inhibitory Effect of Ultrasound on Kiwifruit Bacterial Canker

ZHEN Xi,LIN Shiyan,WANG Naling,ZHANG Zonghao,LI Junjie,TAN Zecheng,LIU Shibiao,ZHA Manrong,WANG Yan

(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)

Online:2024-05-25 Published:2024-06-19

摘要/Abstract

摘要：首先通过设置不同超声频率、超声处理时长在体外对丁香假单胞菌猕猴桃致病变种（Psa）进行处理，检测处理后Psa的活菌数、呼吸链脱氢酶活性，筛选具有较好杀菌效果的超声处理条件，然后对Psa侵染后的湘吉红、贝木离体枝条进行不同条件的超声处理，培养30 d后观测湘吉红、贝木离体枝条溃疡病病斑大小，探究不同超声条件对猕猴桃枝条溃疡病的抑制效果.结果表明，60 kHz超声处理45 min或者80 kHz超声处理15 min能够将贝木枝条病斑控制在2 cm左右，80 kHz超声处理30 min能将湘吉红枝条病斑控制在2 cm内，且能完全抑制贝木枝条的溃疡病感染.猕

关键词： 猴桃溃疡病, 超声杀菌, 病害防治, 丁香假单胞菌猕猴桃致病变种

Abstract: Pseudomonas syringae pv. actinidiae (Psa) was treated in vitro by setting different ultrasonic frequencies and ultrasonic durations.The number of viable bacteria and respiratory chain dehydrogenase activity of Psa were detected after treatment,and the ultrasonic treatment conditions with better bactericidal effect were screened.Secondly,the detached branches of Xiangjihong and Beimu after Psa infection were treated with ultrasonic waves under different conditions.After 30 days of culture,the size of canker lesions was detected to explore the inhibitory effect of different ultrasonic conditions on kiwifruit canker.The results showed that 60 kHz ultrasonic treatment for 45 min or 80 kHz ultrasonic treatment for 15 min could control the lesion of Beimu branches to about 2 cm.Treatment with 80 kHz for 30 min could control the lesion of Xiangjihong branches within 2 cm,and completely inhibit the infection of canker spot on the Beimu branches.

Key words: kiwifruit bacterial canker, ultrasonic sterilization, disease control, Pseudomonas syringae pv. actinidiae

镇熙, 林诗艳, 王娜灵, 张棕豪, 黎骏杰, 谭泽成, 刘世彪, 查满荣, 王琰. 超声对猕猴桃溃疡病的抑制作用[J]. 吉首大学学报（自然科学版）, 2024, 45(3): 29-34.

ZHEN Xi, LIN Shiyan, WANG Naling, ZHANG Zonghao, LI Junjie, TAN Zecheng, LIU Shibiao, ZHA Manrong, WANG Yan. Inhibitory Effect of Ultrasound on Kiwifruit Bacterial Canker[J]. Journal of Jishou University(Natural Sciences Edition), 2024, 45(3): 29-34.

参考文献

[1] EVERETT KERRY R,TAYLOR ROBERT K,ROMBERG MEGAN K,et al.First Report of Pseudomonas syringae pv. actinidiae Causing Kiwifruit Bacterial Canker in New Zealand[J].Plant Disease,2011,6:67-71.
[2] LEE YOUNG SUN,KIM JIN,KIM GYOUNG HEE,et al.Biovars of Pseudomonas syringae pv. actinidiae Strains,the Causal Agent of Bacterial Canker of Kiwifruit,Isolated in Korea[J].Research in Plant Disease,2017,23(1):35-41.
[3] 钟彩虹,李黎,潘慧,等.猕猴桃细菌性溃疡病的发生规律及综合防治技术[J].中国果树,2020,1:9-13;18.
[4] VANNESTE J L,KAY C,ONORATO R,et al.Recent Advances in the Characterisation and Control of Pseudomonas syringae pv. actinidiae,the Causal Agent of Bacterial Canker on Kiwifruit[J].Acta Horticulturae,2011,913:443-455.
[5] FROUD KARYN J,EVERETT KERRR,TYSON JOY L,et al.Review of the Risk Factors Associated with Kiwifruit Bacterial Canker Caused by Pseudomonas syringae pv. actinidiae[J].Insects & Diseases in Apples & Kiwifruit,2015,68:313-327.
[6] RENZI MARSILIO,COPINI PAUL,TADDEI ANNA R,et al.Bacterial Canker on Kiwifruit in Italy:Anatomical Changes in the Wood and in the Primary Infection Sites[J].Bacteriology,2012,102(9):827-840.
[7] 陈虹君,杨国安,迟旭春.猕猴桃溃疡病致病因素与防治经验探讨[J].南方农业,2018,12(17):1-2.
[8] HAN H S,NAM H Y,KOH Y J,et al.Molecular Bases of High-Level Streptomycin Resistance in Pseudomonas arginalis and Pseudomonas syringae pv. acdnidiae[J].Journal of Microbiology,2003,41(1):16-21.
[9] 朱海云,马瑜,柯杨,等.猕猴桃细菌性溃疡病生防菌的筛选、鉴定及其防效初探[J].微生物学杂志，2016,36(5):90-96.
[10] LEGAY MATHIEU,GONDREXON NICOLAS,PERSON STPHANE LE,et al.Enhancement of Heat Transfer by Ultrasound:Review and Recent Advances[J].International Journal of Chemical Engineering,2011,1:1-17.
[11] BRENNAN JAMES G,GRANDISON ALISTAIR S.Food Processing Handbook[M].Hoboken:Wiley-Ych,2006:201-232.
[12] 陶文卿.10-HDA对大肠杆菌抑制机理的研究[D].济南:山东轻工业学院,2012.
[13] 赵军嫄,李子富,周瑱,等.超声波/紫外线一体化反应器用于污水消毒的中试研究[J].环境工程学报,2016,10(11):6185-6189.
[14] 马海燕,张慜,孙金才,等.生冻大根泥(萝卜泥)中微生物控制的物理方法[J].食品与生物技术学报，2011,30(1):78-83.
[15] 贾志成,郑加强,黄雅杰,等.柑橘黄龙病热处理防治技术研究进展[J].农业工程学报,2015,31(23):1-9.
[16] CHANDRAPALA JAYANI,OLIVER CHRISTINE,KENTISH SADRA,et al.Ultrasonics in Food Processing-Food Quality Assurance and Food Safety[J].Trends in Food Science & Technology,2012,26(2):88-98.
[17] 张鑫,刘芸宏,高贵田,等.超声+热处理对猕猴桃枝条组织中溃疡病菌的杀菌作用及对苗木生长的影响[J].果树学报,2019,36(5):638-646.
[18] 李瑶,承河元,方书苗,等.猕猴桃细菌性溃疡病流行预测初探[J].应用生态学报,2001,12(3):355-358.
[19] 韩明丽,张志友,陈丽萍,等.猕猴桃溃疡病发生的影响因素及其防治方法[J].湖南农业学报,2013,21:77-80.

超声对猕猴桃溃疡病的抑制作用

Inhibitory Effect of Ultrasound on Kiwifruit Bacterial Canker

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