溃疡病猕猴桃和健康猕猴桃根际土壤细菌群落结构

doi:10.13438/j.cnki.jdzk.2023.01.009

吉首大学学报（自然科学版） ›› 2023, Vol. 44 ›› Issue (1): 59-67.DOI: 10.13438/j.cnki.jdzk.2023.01.009

溃疡病猕猴桃和健康猕猴桃根际土壤细菌群落结构

杨睿，姚迪，唐蕴哲，张婧一，彭清忠，汪琳罗沙

（吉首大学生物资源与环境科学学院，湖南吉首 416000）

出版日期:2023-01-25 发布日期:2023-04-10
通讯作者: 汪琳罗沙（1996—），女，湖南吉首人，吉首大学生物资源与环境科学学院硕士研究生，主要从事微生物学研究.E-mail:985906935@qq.com.
作者简介:杨睿（1995—），男，湖南怀化人，吉首大学生物资源与环境科学学院硕士研究生，主要从事微生物生态学研究.E-mail:1250937585@qq.com
基金资助:
国家自然科学基金资助项目（31260009）；湖南省重点研发计划项目（2022NK2055）

Analysis of the Rhizosphere Soil Bacterial Community Composition of Kiwifruits with Canker Disease and Healthy Kiwifruits

YANG Rui,YAO Di,TANG Yunzhe,ZHANG Jingyi,PENG Qingzhong,WANG Linluosha

(College of Biology and Environmental Sciences,Jishou University,Jishou 416000,Hunan China)

Online:2023-01-25 Published:2023-04-10

摘要/Abstract

摘要：从湘西永顺县猕猴桃基地分别采集萌芽期（3月）、生长期（6月）和成熟期（9月）溃疡病猕猴桃和健康猕猴桃根际土壤样品，采用宏基因组测序技术探究了土壤样品中细菌群落结构和种群丰度.结果发现：虽然3个时期健康猕猴桃和病患猕猴桃根际土壤的细菌群落结构和种群丰度存在一定差异，但优势属主要包括Acidobacteria类群、芽单胞菌属、Gaiella等.其中假单胞菌属细菌的种群丰度随生长期延长呈现出先上升后下降的规律，与猕猴桃溃疡病发生进程吻合，提示假单胞菌属细菌可能是猕猴桃溃疡病发生的传染源.此外，黄杆菌科和丛毛单胞菌科细菌在病患猕猴桃土壤样品中丰度高于健康样品，推测其与猕猴桃溃疡病的发生存在正相关关系；红螺菌科、高温单孢菌科，芽孢杆菌属和链霉菌属细菌在健康猕猴桃土壤样品中丰度高于病患样品，推测其在猕猴桃溃疡病发生中起拮抗作用.

关键词： 猕猴桃溃疡病, 根际土壤, 宏基因组测序, 细菌群落结构

Abstract: In this work,the rhizosphere soil samples at three stages of kiwifruit development were collected from the kiwifruit planting base in Yongshun County,West Hunan.The bacterial community composition and population abundance in the canker and healthy samples were analyzed by metagenomic sequencing.The results showed that there were some differences in the bacterial community composition and population abundance in three periods of rhizosphere soil samples of the healthy and diseased kiwifruit,but the dominant bacteria mainly included AcidobacteriaGp,Gemmatimonas and Gaiella.The population abundance of Pseudomonas increased and then decreased with three stages of kiwifruit development,coinciding with the process of kiwifruit canker disease,which indicates that the rhizosphere soil bacterial community is one of the infectious sources for the kiwifruit canker occurrence.In addition,it was found the abundance of flavobacteriaceae and comamonadaceae was higher in the canker samples than in the healthy samples,presumably positively correlated with the occurrence of kiwifruit canker disease.The abundances of Rhodospirillaceae,Thermomonosporaceae,Bacillus and Streptomyces were higher in the healthy samples than in the diseased samples,presumably playing an antagonistic role in the occurrence of kiwifruit canker disease.

Key words: kiwifruit canker disease, rhizosphere soil, metagenomic sequencing, bacterial community composition

杨睿, 姚迪, 唐蕴哲, 张婧一, 彭清忠, 汪琳罗沙. 溃疡病猕猴桃和健康猕猴桃根际土壤细菌群落结构[J]. 吉首大学学报（自然科学版）, 2023, 44(1): 59-67.

YANG Rui, YAO Di, TANG Yunzhe, ZHANG Jingyi, PENG Qingzhong, WANG Linluosha. Analysis of the Rhizosphere Soil Bacterial Community Composition of Kiwifruits with Canker Disease and Healthy Kiwifruits[J]. Journal of Jishou University(Natural Sciences Edition), 2023, 44(1): 59-67.

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溃疡病猕猴桃和健康猕猴桃根际土壤细菌群落结构

Analysis of the Rhizosphere Soil Bacterial Community Composition of Kiwifruits with Canker Disease and Healthy Kiwifruits

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