吉首大学学报(自然科学版) ›› 2022, Vol. 43 ›› Issue (5): 47-51.DOI: 10.13438/j.cnki.jdzk.2022.05.008

• 药物分析 • 上一篇    下一篇

HPLC法测定复方桐叶烧伤油中芝麻素的含量

覃爱桃,杨德泉,韩湘云,徐玉辉   

  1. (1.湘西土家族苗族自治州食品药品检验所,湖南 吉首 416000;2. 湘西宏成制药有限责任公司,湖南 吉首 416000)
  • 出版日期:2022-09-25 发布日期:2023-01-11
  • 通讯作者: 杨德泉(1963—),男,湖南常德人,湘西土家族苗族自治州食品药品检验所主任药师,主要从事中药检验技术和民族药标准研究.E-mail:1198707038@qq.com.
  • 基金资助:
    湖南省自然科学基金资助项目(2020JJ9053)

Determination of Sesamin in Compound Tongye Burn Oil by HPLC

QIN Aitao,YANG Dequan,HAN Xiangyun,XU Yuhui   

  1. (1.Food and Drug Inspection Institute,Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan China;2.China Hunan Hongcheng Pharmaceutical Co.LTD,Jishou 416000,Hunan China)
  • Online:2022-09-25 Published:2023-01-11

摘要:利用HPLC法(高效液相色谱分析)测定了复方桐叶烧伤油中芝麻素的含量.实验色谱条件为:TADE-PAK AF-C18色谱柱(250 mm×4.6 mm,5  μm),流动相甲醇-水(体积比80∶20),流速0.8 mL/min,检测波长287 nm.柱温35 ℃,进样量10   μL.理论板数按芝麻素计算,不低于5 000.实验结果表明,芝麻素对照品在0.020 18 ~0.403 6 g/L范围内线性关系良好(R=1),回归方程Y=15 562X+9.37,芝麻素加样回收率103.22%,精密度RSD 0.58%.方法简单、快速、准确、灵敏、稳定,可用于复方桐叶烧伤油中芝麻素含量的检测和质量控制.

关键词: 复方桐叶烧伤油, 芝麻素, 麻油, HPLC

Abstract: A method for the determination of sesamin in compound tongye burn oil by high performance liquid chromatography (HPLC) was established.The chromatographic conditions were:tade-pak af-c18 column (250 mm×4.6 mm,5  μm);mobile phase:methanol water (80∶20);flow rate:0.8 mL/min;detection wavelength:287 nm;column temperature:35 ℃;injection volume:10  μL.The number of theoretical plates shall be calculated as sesamin and shall not be less than 5 000.The linearity of sesamin reference substance was good in the range of 0.020 18~0.403 6 g/L (R=1),the regression equation was Y=15 562X+ 9.37,the recovery of sesamin was 103.22%,and the precision RSD was 0.58%.The results show that the method is simple,rapid,accurate,sensitive and stable,and can be used for the content detection and quality control of sesamin in compound tongye burn oil.

Key words: compound tongye burn oil, sesamin, sesame oil, HPLC

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