吉首大学学报（自然科学版）

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大承气汤对支气管哮喘及肺肠合病Treg/TH17免疫机制的作用

李继红，王小莉，霍吉尔，吴秀明   

  1. (1.吉首大学医学院，湖南 吉首 416000；2.张家界市人民医院呼吸内科，湖南 张家界 427000)
  • 出版日期:2019-07-25 发布日期:2019-07-31
  • 通讯作者: 吴秀明（1976—），女（苗族），湖南花垣人，张家界市人民医院呼吸内科主任医师，医学博士，主要从事呼吸内科研究.E-mail:wuxiuming2000@163.com.
  • 基金资助:

    国家自然科学基金资助项目（81260008）；湖南省教育厅科学研究项目（13C763）

Effects of Dachengqi Decoction on the Immunologic Mechanism of TH17/Treg in Bronchial Asthma and Lung-Intestinal Disease

LI Jihong, WANG Xiaoli, HUO Jier, WU Xiuming   

  1. (1. College of Medicine,  Jishou University,  Jishou 416000,  Hunan China; 2.  Respiratory Medicine Department,  Zhangjiajie People's Hospital,  Zhangjiajie 427000,  Hunan China)
  • Online:2019-07-25 Published:2019-07-31

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摘要：

目的 从“肺与大肠相表里”理论出发，以大承气汤通肠利肺作用为切入点，观察支气管哮喘（简称哮喘）及肺肠合病大鼠动物模型及在大承气汤方药干预下，肺与大肠之间共同免疫物质TH17/Treg的变化，探讨“肺与大肠相表里”的免疫机制.方法 建立哮喘组、肺肠合病组、大承气汤组哮喘干预组与肺肠合病干预组动物模型，检测各组大鼠外周血淋巴细胞中Th17/Treg亚群分布表达,及血清中 IL-17，IL-6，IL-2的水平.结果 与正常对照组比较，哮喘组及肺肠合病组大鼠外周血中Th17细胞比例升高，Treg细胞比例降低，血清中IL-6，IL-17水平升高，IL-2水平降低，有差异具有统计学意义（P＜0.005）；肺肠合病组大鼠组更明显（P＜0.005）.在大承气汤干预下，与哮喘组比较，哮喘干预组与肺肠合病干预组Th17细胞比例降低，Treg细胞比例升高，血清IL-6，IL-17水平下降，IL-2水平升高，差异有统计学意义（P＜0.005）.结论 哮喘发病时肠道功能的变化可能导致肺、肠TH17/Treg的免疫失衡，免疫反应向呼吸道粘膜等部位迁移，加重哮喘的发病.大承气汤是可以通过调节肠道TH17/Treg免疫，提高黏膜屏障功能，降低淋巴细胞的“归巢”作用，减轻哮喘发病.

关键词： 支气管哮喘, 肺肠合病, TH17/Treg, 大承气汤

Abstract:

TH17/Treg are common immune substances between lung and large intestine.  To explore the immune mechanism of "interior-exterior relationship between lung and large intestine",  the study has observed the changes of TH17/Treg in the rat model with bronchial asthma (asthma) and lung-intestinal disease.  Asthma group,  lung-intestinal disease group,  and Dachengqi decoction group of rat models were established.  Dachengqi decoction group was divided into asthma intervention group and lung-intestinal disease intervention group.  The distribution and expression of Th17 and Treg subsets in peripheral blood lymphocytes and the levels of IL-17,  IL-6 and IL-2 in serum of rats in each group were detected.  The results showed that,  compared with the normal control group,  the proportion of Th17 cells increased and that of Treg cells decreased,  IL-6 and IL-17 levels increased and IL-2 levels decreased in serum of asthma group and lung-intestinal disease group (P<0.005),  especially in lung-intestinal disease group (P<0.005).  With the intervention of Dachengqi decoction,  compared with asthma group,  the proportion of Th17 cells decreased,  that of Treg cells increased,  the levels of IL-6 and IL-17 decreased,  and the level of IL-2 increased in serum of asthma intervention group and lung-intestinal disease intervention group (P<0.005).  It can be concluded that the changes of intestinal immune function may lead to imbalance of TH17/Treg in asthma.  Migration of immune response to respiratory mucosa and other parts can aggravate the onset of asthma.  Dachengqi decoction can alleviate asthma by regulating intestinal TH17/Treg immunity,  which can improve the mucosal barrier function and reduce lymphocyte "homing".

Key words: bronchial asthma, lung-intestinal disease, TH17/Treg, Dachengqi decoction

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