利用大孔树脂从啤酒废酵母中分离谷胱甘肽

journal6 ›› 2008, Vol. 29 ›› Issue (1): 83-86.

利用大孔树脂从啤酒废酵母中分离谷胱甘肽

（1.湖北工业大学生物工程学院，湖北武汉 430068；2.山东齐鲁制药有限公司，山东济南 250000）

出版日期:2008-01-25 发布日期:2012-05-26
作者简介:邱雁临（1942-），女，湖北工业大学生物工程学院教授，博士生导师，主要从事生化技术及再生资源的利用研究.
基金资助:
湖北省教育厅重大科学研究项目（2003Z001）

Separation of Glutathione from Abandoned Beer Yeast by Macroporous Resin

(1.College of Bioengineering,Hubei University of Technology,Wuhan 430068,China;2.Shangdong Qilu Pharmacy Ctd. Co.,Jinan 250000,China)

Online:2008-01-25 Published:2012-05-26

摘要/Abstract

摘要：利用大孔树脂D001分离纯化还原型谷胱甘肽，其最佳试验条件为：pH值4.5、浓度为0.02 mol/L的磷酸缓冲液平衡，pH值7.5、浓度为0.02 mol/L的磷酸缓冲液洗脱，洗脱流速为2.0 mL/min.此法所得到的粗品颜色为白色，经高效液相色谱分析，其GSH质量分数为28.47%,平均收率为71.6%.

关键词： 谷胱甘肽, 大孔树脂D001, 啤酒废酵母

Abstract: Macroporous resin D001 was adopted for separation of GSH from abandoned beer yeast.The result indicated that optimum technological parameters of separation was that pH of 0.02 mol/L phosphate buffer was 4.5 for adsorption ,while 7.5 for elution,and the elution ratio was 2.9 mL/min.The raw product was white,the purity of the GSH was 28.47% analysised by HPLC,and the reclaimed ratio was 71.6%.

Key words: glutathione, macroporous resin, abandoned beer yeast

邱雁临, 胡静, 缪谨枫, 梁亮. 利用大孔树脂从啤酒废酵母中分离谷胱甘肽[J]. journal6, 2008, 29(1): 83-86.

QIU Yan-Lin, HU Jing, MIAO Jin-Feng, LIANG Liang. Separation of Glutathione from Abandoned Beer Yeast by Macroporous Resin[J]. journal6, 2008, 29(1): 83-86.