吉首大学学报(自然科学版) ›› 2024, Vol. 45 ›› Issue (6): 68-71.DOI: 10.13438/j.cnki.jdzk.2024.06.010

• 药物分析与鉴别 • 上一篇    下一篇

球药隔重楼根茎与延龄草根茎及重楼的薄层色谱鉴别

杨德泉,佘永红   

  1. (湘西土家族苗族自治州食品药品检验所,湖南 吉首 416000)
  • 出版日期:2024-11-25 发布日期:2024-12-19
  • 作者简介:杨德泉(1963—),男,湖南常德人,湘西土家族苗族自治州食品药品检验所主任药师,主要从事中药民族药检验技术和质量标准研究.
  • 基金资助:
    湖南省自然科学基金资助项目(2022JJ80032)

Thin-Layer Chromatography Identification of Paris fargesii Rhizome,Trillium tschonoskii Rhizome,and Paris Rhizome

YANG Dequan,SHE Yonghong   

  1. (Food and Drug Inspection Institute,Xiangxi Tujia and Miao Autonomous Prefecture,Jishou 416000,Hunan China)
  • Online:2024-11-25 Published:2024-12-19

摘要:以重楼皂苷II,VI,H为对照品,采用薄层色谱方法,对球药隔重楼根茎、延龄草根茎和重楼(七叶一枝花和云南重楼的根茎)进行了鉴别.结果表明,采用硅胶G薄层板,以三氯甲烷-甲醇-水(体积比15∶5∶1)作为展开剂,在20 ℃±2 ℃、湿度50%~60%条件下,3种对照品分离度高,斑点清晰,并根据成分差异,能对球药隔重楼根茎、延龄草根茎和重楼进行薄层色谱鉴别.

关键词: 球药隔重楼根茎, 延龄草根茎, 重楼, 重楼皂苷Ⅱ, 重楼皂苷Ⅵ, 重楼皂苷H, 薄层色谱鉴别

Abstract: Paris Saponin II,VI,H were used as reference substances,and the rhizomes of Paris fargesii,Trillium tschonoskii,and Paris (The rhizome of Paris polyphylla Smith var. chinensis (Franch.) Hara and Paris polyphylla Smith var. yunnanensis (Franch.) Hand.-Mazz.) were identified by thin-layer chromatography (TLC).The results showed that the three reference substances had high resolution and clear spots on the silica gel G thin-layer plate with chloroform-methanol-water (15∶5∶1) as the developing agent under the conditions of 20 ℃±2 ℃ and 50%~60%.According to the difference in components,TLC can be used to distinguish Paridis fargesii rhizome,Trillium tschonoskii rhizome,and Paris rhizome.

Key words: Paridis fargesii rhizome, Trillium tschonoskii rhizome, Paris rhizome, Paris Saponin Ⅱ, Paris Saponin Ⅵ, Paris Saponin H, thin layer chromatography

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