LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用

doi:10.13438/j.cnki.jdzk.2021.06.008

吉首大学学报（自然科学版） ›› 2021, Vol. 42 ›› Issue (6): 48-53.DOI: 10.13438/j.cnki.jdzk.2021.06.008

LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用

朱长明，孙高，陈功华，龚于刚，夏杰

（1.吉首大学附属张家界市人民医院胃肠外科，湖南张家界 427000；2.吉首大学医学院，湖南吉首 416000）

出版日期:2021-11-25 发布日期:2022-01-21
作者简介:朱长明（1980—），男，湖南张家界人，厦门大学外科学硕士研究生毕业，主要从事胃肠肿瘤研究.

Effect of LncRNA-ROR on the Hypoxia-Induced Gastric Cancer Metastasis and Invasion

ZHU Changming, SUN Gao, CHEN Gonghua, GONG Yugang, XIA Jie

(1.Department of Gastrointestinal Surgery, Zhangjiajie People's Hospital Affiliated to Jishou University, Zhangjiajie 427000, Hunan China; 2. School of Medical, Jishou University, Jishou 416000, Hunan China)

Online:2021-11-25 Published:2022-01-21

摘要/Abstract

摘要：目的探讨LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用.方法采用RT-PCR法检测胃癌组织标本和胃癌细胞株中ROR mRNA的表达差异；通过基因重组方法构建ROR mRNA过表达载体，并转染SGC-7901和MGC-803胃癌细胞，再使用RT-PCR法检测过表达转载效果；通过Transwell试验研究缺氧环境下LV5-ROR对胃癌细胞SGC-7901和MGC-803侵袭及转移能力的影响.结果 ROR mRNA在胃癌组织中的表达低于癌旁组织，同时，在缺氧诱导下胃癌细胞株中的ROR mRNA表达明显下降；缺氧能够促进SGC-7901和MGC-803细胞的侵袭及转移，而过表达ROR mRNA能够明显抑制缺氧条件下SGC-7901及MGC-803细胞的侵袭及转移.结论 LncRNA-ROR能抑制胃癌细胞的侵袭及转移，为胃癌靶向治疗提供理论依据.

关键词： 胃癌, ROR, 长链非编码RNA, 侵袭转移

Abstract: Objective To investigate the effect of LncRNA ROR on metastasis and invasion in the hypoxia-induced invasion and metastasis in GC.Methods Using quantitative real-time polymerase chain reaction (RT-PCR) to detect the expression of ROR ingastric cancer tissues and cell lines.Employing the over-expression technique were used to observe the effect of overexpression of ROR on human gastric cancer cell metastasis and invasion, the transfection efficiency was verified by RT-PCR in SGC-7901 and MGC-803.Results ROR was significantly down-regulated in gastric cancer tissues samples compared with adjacent normal gastric tissues, and also down-regulated inthe hypoxic—induced GC cells. Hypoxia significantly increased the migration and invasion potential of SGC-7901 and MGC-803，After over-expression of ROR the hypoxia-induced migration and invasion of SGC7901 and MGC-803 cells were dramatically diminished. Conclusion LncRNA-ROR expression is decreased in hypoxic GC and suppressed hypoxia-induced GC metastasis and invasion, which could be a new biomarkers, a potential therapeutic target of gastric cancer.

Key words: gastric cancer, ROR, gastric cancer, metastasis and invasion

朱长明, 孙高, 陈功华, 龚于刚, 夏杰. LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用[J]. 吉首大学学报（自然科学版）, 2021, 42(6): 48-53.

ZHU Changming, SUN Gao, CHEN Gonghua, GONG Yugang, XIA Jie. Effect of LncRNA-ROR on the Hypoxia-Induced Gastric Cancer Metastasis and Invasion[J]. Journal of Jishou University(Natural Sciences Edition), 2021, 42(6): 48-53.

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LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用

Effect of LncRNA-ROR on the Hypoxia-Induced Gastric Cancer Metastasis and Invasion

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