吉首大学学报(自然科学版)

• 化学化工 • 上一篇    下一篇

毛细管电泳法检测饮料中的防腐剂

陈林情,杨昆,苏安梅,钟青梅,王益林   

  1. (1.广西大学化学化工学院,广西生物炼制重点实验室,广西 南宁 530004;2.广西出入境检验检疫局技术中心,广西 南宁 530028)
  • 出版日期:2017-11-25 发布日期:2017-12-29
  • 通讯作者: 王益林,男,广西大学化学化工学院副教授,博士,主要从事毛细管电泳分析研究.
  • 基金资助:

    广西自然科学基金资助项目(2014GXNSFAA118328);广西生物炼制重点实验室开放基金资助项目(GXKLB-2)

Detection of Preservatives in Beverages Through Capillary Electrophoresis

CHEN Linqing,YANG Kun,SU Anmei,ZHONG Qingmei,WANG Yilin   

  1. (1.Guangxi Key Laboratory of Biorefinery,School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,Guangxi China;2.Technology Center,Entry-Exit Inspection and Quarantine Bureau of Guangxi,Nanning 530028,Guangxi China)
  • Online:2017-11-25 Published:2017-12-29

摘要:

建立了毛细管电泳同时测定山梨酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯4种防腐剂的分析方法.以20 mmol/L硼酸-硼酸钠(pH值10.0)为缓冲液,在有效长度为41 cm、内径为50 μm的石英毛细管中,当分离电压为15 kV时,4种防腐剂在9 min内完全分离.在该条件下,分析物的峰面积与其质量浓度在10~1000 mg/L范围内呈良好的线性关系,回收率范围为95.4%~103.7%,多次测定结果的相对标准偏差小于4.5 %.实验表明,该方法应用于饮料中山梨酸、对羟基苯甲酸乙酯和对羟基苯甲酸丙酯的分离检测时,简单快速且结果可靠.

关键词: 毛细管电泳, 防腐剂, 饮料, 测定

Abstract:

A method for simultaneous detection of sorbic acid,methyl-p-hydroxybenzoate,ethyl-p-hydroxybenzoate,propyl-p-hydroxybenzoate through capillary electrophoresis was developed.With the composition of 20 mmol/L H3BO3-Na2B4O7 (pH=10.0) as the buffer and in a capillary with an effective length of 41cm and an inner diameter of 50  μm,four kinds of preservatives were separated at a voltage of 15 kV within 9 minutes.Under these conditions,the analyte peak area was linearly proportional to its concentration within the range of 10 ~ 1 000 mg/L,the recovery was 95.4%~103.7%,and the relative standard deviation was less than 4.5%.The experiments show that this method is a desirable approach for the analysis of preservatives in beverage.

Key words: capillary electrophoresis, preservatives, beverages, detection

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