溶藻弧菌vscO基因在不同环境下的表达差异

doi:10.3969/j.issn.1007-2985.2014.04.014

journal6 ›› 2014, Vol. 35 ›› Issue (4): 59-63.DOI: 10.3969/j.issn.1007-2985.2014.04.014

溶藻弧菌vscO基因在不同环境下的表达差异

庞欢瑛,周泽军,丁燏,黄郁葱,吴灶和,简纪常

（1.广东海洋大学水产学院，广东湛江 524088；2.广东省水产经济动物病原生物学及流行病学重点实验室，广东湛江 524088；3.广东省教育厅水产经济动物病害控制重点实验室，广东湛江 524088；4.仲恺农业工程学院，广东广州 510225）

出版日期:2014-07-25 发布日期:2014-07-21
通讯作者: 简纪常（1964-），男，广东海洋大学水产学院教授，博士，主要从事水产经济动物免疫学及病害控制研究，E-mail jianjc@gmail.com
作者简介:庞欢瑛（1980-），女，广西北海人，广东海洋大学水产学院讲师，博士，主要从事水产经济动物病害研究
基金资助:
国家科技支撑计划项目（2012BAD17B02,2012BAD17B03）；广东省自然科学基金资助项目（S2013040014562）

Different Expressions of vscO Gene from Vibrio Alginolyticus in Different Environments

PANG Huan-Ying, ZHOU Ze-Jun, DING Yu, HUANG Yu-Cong, WU Zao-He, JIAN Ji-Chang

(1.Fisheries College of Guangdong Ocean University,Zhanjiang 524088,Guangdong China;2.Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals,Zhanjiang 524088,Guangdong China;3.Key Laboratory of Diseases Controlling for Aquatic Economic Animals,Guangdong Higher Education Institutions,Zhanjiang 524088,Guangdong China;4.Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China)

Online:2014-07-25 Published:2014-07-21

摘要/Abstract

摘要：为阐明溶藻弧菌vscO的在不同环境下的表达情况，用半定量RT-PCR方法检测vscO mRNA在不同生长时期、不同环境因子（温度、盐度和pH值）培养下的相对表达量.结果表明，溶藻弧菌vscO在对数期，温度为28 ℃，盐度为2%以及培养基起始pH值为7.0时的表达量最高.

关键词： 溶藻弧菌, Ⅲ型分泌系统（T3SS）, vscO基因, 环境因子, 基因表达

Abstract: To understand the impact of environmental factors on the expressions of vscO gene,the mRNA expressions of vscO gene in different growth periods and with environmental factors,such as temperature,salinity and pH,were detected by RT-PCR method. The results indicated that the mRNA expression of vscO reached a maximum at 28 ℃ in salinity of 2%,with the initial medium pH 7.0 when V. alginolyticus was in the middle phase of growth.

Key words: Vibrio alginolyticus, T3SS, vscO gene, environmental factors, gene expression

庞欢瑛, 周泽军, 丁燏, 黄郁葱, 吴灶和, 简纪常. 溶藻弧菌vscO基因在不同环境下的表达差异[J]. journal6, 2014, 35(4): 59-63.

PANG Huan-Ying, ZHOU Ze-Jun, DING Yu, HUANG Yu-Cong, WU Zao-He, JIAN Ji-Chang. Different Expressions of vscO Gene from Vibrio Alginolyticus in Different Environments[J]. journal6, 2014, 35(4): 59-63.

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溶藻弧菌vscO基因在不同环境下的表达差异

Different Expressions of vscO Gene from Vibrio Alginolyticus in Different Environments

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