红檵木幼叶的水和醇水提取物抑菌活性分析

doi:10.3969/j.issn.1007-2985.2012.05.020

journal6 ›› 2012, Vol. 33 ›› Issue (5): 78-81.DOI: 10.3969/j.issn.1007-2985.2012.05.020

红檵木幼叶的水和醇水提取物抑菌活性分析

(湖南省林产化工工程重点实验室，湖南张家界 427000)

出版日期:2012-09-25 发布日期:2012-11-30
作者简介:赵虹桥（1972-），男，湖南张家界人，吉首大学张家界校区林产化工工程重点实验室副教授，主要从事植物活性成分研究.
基金资助:
湖南省教育厅科学研究资助项目（S2009F2072）

Bacteriorstasis of the Pigments Extracted from the L. Chinense Var. Rubrum Leaf

(Key Laboratory for Forest Products Chemical Industry Engineering of Hunan,Jishou University,Zhangjiajie 427000,Hunan China)

Online:2012-09-25 Published:2012-11-30

摘要/Abstract

摘要：用蒸馏水和20%乙醇经微波辅助处理提取红檵木幼叶成分，对该水和醇水的提取物进行浓缩或萃取精制，采用牛津杯法测定水和醇水提取物样液对3种细菌的抑制效果，并用平板稀释法测定其最小抑菌浓度.幼叶水提取液对3种细菌都有明显的抑制作用，抑菌圈较大，对大肠杆菌、金黄色葡萄球菌和痢疾杆菌的最小抑菌生药质量浓度分别为 0.014，0.010，0.010 g/mL；幼叶的醇提取液对大肠杆菌的抑制效果不明显，对金黄色葡萄球菌和痢疾杆菌有较强抑制作用,其最小抑菌生药质量浓度分别为0.011，0.014 g/mL.

关键词： 红檵木, 抑菌活性, 最小抑菌生药浓度（MIC）

Abstract: The pigment in the browse leaves was extracted from L. Chinese Var. Rubrum leaf with Microwave treatment and distilled water or 20% EtOH.The antibacterial activities of the extracts on the three kinds bacteria were determined by Oxford Cup method.If the extracts can abstrain the bacteria obviously,the MIC of crude drug were determined by microdilution technique.The results show that the extracts from browse leaves by distilled water under microwave treatment have obviously antimicrobial activity on three kinds bacteria,the MIC of crude drug of the extracts from browse leaves on E. coli,shigella dysenteroae,staphylococlus aureus are 0.014 g/mL,0.010 g/mL,and 0.010 g/mL.The extracts from browse leaves by 20% EtOH were obviously active against staphylococlus aureus and shigella dysenteroae but can not abstrain the E.coli.

Key words: L. chinense Var.rubrum, antibaterial action, minimal inhibition concentration of crude drug (MIC)

赵虹桥, 卢成瑛. 红檵木幼叶的水和醇水提取物抑菌活性分析[J]. journal6, 2012, 33(5): 78-81.

ZHAO Hong-Qiao, LU Cheng-Ying. Bacteriorstasis of the Pigments Extracted from the L. Chinense Var. Rubrum Leaf[J]. journal6, 2012, 33(5): 78-81.

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红檵木幼叶的水和醇水提取物抑菌活性分析

Bacteriorstasis of the Pigments Extracted from the L. Chinense Var. Rubrum Leaf

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