黄瓜花叶病毒感染引起甜瓜植株苯丙氨酸解氨酶和叶绿素的变化

journal6 ›› 2006, Vol. 27 ›› Issue (3): 78-81.

黄瓜花叶病毒感染引起甜瓜植株苯丙氨酸解氨酶和叶绿素的变化

(北京师范大学生命科学学院，北京 100875)

出版日期:2006-05-25 发布日期:2012-09-11
作者简介:赵荣乐（1945－），女，江苏省徐州人，北京师范大学大学生命科学学院教授，主要从事生物化学与分子生物学研究.
基金资助:
国家自然科学基金资助项目(3880581)；教育部留学回国人员科研基金资助项目

Changes of Phenylalanine Ammonialyase and Chlorophyll in Muskmelon Plants Infected by CMV-Xinjiang Isolate

(College of Life Sciences,Beijing Normal University,Beijing 100875,China)

Online:2006-05-25 Published:2012-09-11

摘要/Abstract

摘要：研究黄瓜花叶病毒新疆株(cucumber mosaic virus-XJ，CMV-2-XJ)感染抗性不同的甜瓜品种后苯丙氨酸解氨酶和叶绿素含量的变化，结果表明：与健康对照相比，受感染植株的酶活性均有所增高，但抗性品种“新玉”增高的幅度是感性品种“网纹香”的2倍，酶活性峰早出现12 d，即抗性品种酶活性增高幅度大，酶活性峰出现早；叶绿素的变化则与抗性呈负相关，抗性品种“新玉”受CMV-XJ感染后的叶绿素含量与健康对照差异不大，而感性品种“网纹香”的叶绿素含量则比健康对照下降了62.9%.说明苯丙氨酸解氨酶和叶绿素含量的变化均与甜瓜品种对CMV-2-XJ的抗性呈现规律性的相关性，这种规律性可用于甜瓜品种对CMV-XJ抗性鉴定的生物化学分析.

关键词： 黄瓜花叶病毒新疆株(CMV-2), 甜瓜, 苯丙氨酸解氨酶, 叶绿素

Abstract: Changes of phenylalanine ammonialyase and chlorophyll in muskmelon cultivars of different resistance to cucumber mosaic virus-XJ (CMV-XJ) were investigated.Compared with healthy control,the enzyme activity increased in all infected muskmelon plants,but the increase in enzyme activity for resistant cultivar “Xinyu” was two times of that for susceptible cultivar “Wangwenxiang” and the peak of enzyme activity for “Xinyu” appeared 12 days earlier,that is bigger increase range of enzyme activity and earlier enzyme activity peak for resistant cultivar.But the change of chlorophyll was negatively related with the resistance to the virus;after infection by CMV-XJ,the chlorophyll content of resistant cultivar “Xinyu” is not different significantly from that of the healthy control,but the chlorophyll content of susceptible cultivar “Wangwenxiang” decreased by 62.9% compared with the healthy control.There is regular relation between resistance of the muskmelon cultivar and changes of phenylalanine ammonilyase and chlorophyll after infection by CMV-XJ.The regularity can be used as the biological criterion for the determination of susceptibility and resistance to CMV-XJ for muskmelon cultivars.

Key words: cucumber mosaic virus-XJ, muskmelon, phenylalanine ammonialyase, chlorophyll

赵荣乐. 黄瓜花叶病毒感染引起甜瓜植株苯丙氨酸解氨酶和叶绿素的变化[J]. journal6, 2006, 27(3): 78-81.

ZHAO Rong-Le. Changes of Phenylalanine Ammonialyase and Chlorophyll in Muskmelon Plants Infected by CMV-Xinjiang Isolate[J]. journal6, 2006, 27(3): 78-81.