journal6 ›› 2001, Vol. 22 ›› Issue (3): 16-21.

• 博士论坛 • 上一篇    下一篇

林木RAPD标记技术研究进展

  

  1. ( 中南林学院经济林育种与栽培重点实验室, 湖南 株洲 412006)
  • 出版日期:2001-09-15 发布日期:2013-01-05
  • 作者简介:张日清( 1959- ) ,男, 湖南醴陵人,博士研究生, 中南林学院资源与环境学院教授, 主要从事经济林研究.

RAPD Marking and Its Application in Forest Genetics and Tree Breeding

  1. (Ministerial Key Lab of Economic Forest Tree Breeding and Cultivation, South Central Forestry University, Zhuzhou 412006, Hunan China)
  • Online:2001-09-15 Published:2013-01-05

摘要:综述了RAPD该标记在国内外林木遗传图谱构建、群体遗传多样性分析、系统进化研究、品种分类鉴定、DNA指纹分析和性状遗传品质改良等方面的研究进展和发展趋势, 指出随着理论和实验技术研究的不断深入, 分子标记技术将使林业生产迈上新的台阶.

关键词: RAPD标记, 林木遗传育种, 研究进展

Abstract: RAPD marking is derived from PCR methodology and procedures. It is similar to RFLP, SSR and AFLP to some extent but is obviously different from them in many aspects. One disadvantage of RAPD marking exists in the relatively lo wreproducibility, but its reliability as genetic markers has been verified in alarge number of e xperiments.Optimizing the lab conditions and experimental protocols has proved to be instrumental in promoting the results reproducibility. RAPD marking has been extensively used in forest genetic studies and tree breeding trials, including construction of genetic linkage maps, analysis of population genetic st ructure and diversity, study of evolution systems,taxonomic identification, DNA fingerprinting and improvement of genetic traits.

Key words: RAPD marking, forest genetics and tree breeding, literature review

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