原发性肝癌组织中MC与MV计数关系

journal6 ›› 2006, Vol. 27 ›› Issue (5): 113-116.

原发性肝癌组织中MC与MV计数关系

(1.吉首大学医学院，湖南吉首 416000；2.中南大学湘雅二医院肝胆研究室，湖南长沙 410011)

出版日期:2006-09-25 发布日期:2012-06-29
作者简介:向志钢（1969-），男(土家族)，湖南凤凰人，吉首大学医学院副教授，硕士，主要从事外科学教学及临床肝胆胰疾病研究.
基金资助:
湖南省教育厅科学研究项目（05C136）

Study on the Relationship Between the Counts of MC and MV in Tissues of Primary Hepatocarcinoma（PHC）

（1.College of Medicine Jishou University,Jishou 416000,Hunan China;2.Research Lab of Hepatorbiliary Diseases,The Second Xiangya Hospital,Central South University,Changsha 410011,China）

Online:2006-09-25 Published:2012-06-29

摘要/Abstract

摘要：目的研究原发性肝癌PHC）组织中肥大细胞（MC）和微血管（MV）计数及其临床病理意义，探讨二者之间的相互关系.方法应用ABC免疫组化法对47例PHC手术切除标本常规石蜡包埋切片分别检测MV和MC，并在高倍镜下计数.结果 47例PHC癌组织中MV、MC计数均显著高于癌旁组织（MV：67.30±13.68 vs.37.20±10.58,p<0.01;MC：15.46±4.54 vs.8.08±2.03,p<0.01）；术前AFU（α-岩藻糖）≤10　μg/mL的病例及伴转移病例癌组织MV计数显著高于AFU>10 μg/mL病例及无转移病例（AFU：70.41±13.03 vs.59.97±12.69，p<0.05；转移：73.50±13.77个/HP vs.64.10±12.68，p<0.05），MC和MV在PHC中的计数与PHC其他主要临床病理特征无明显关系；癌组织中MC计数与MV计数呈密切正相关（r=0.348，p<0.05）.结论 MC，MV计数与PHC的发生、进展关系密切，且MC具有显著的促血管生成作用.

关键词： 原发性肝癌, 微血管, 巨噬细胞, 计数, 免疫组织化学

Abstract: Objective：To study on the counts of microvessel(MV) and mast cell(MC) in the tissues of primary hepatocarcinoma(PHC) and detect the relation to the clinicopathological features of PHC,and the relationship between the counts of MC and MV.Methods：The stains of MV and MC were completed by the immunohistochemical method of avidin-biotin complex in routine paraffin-embedded sections from 47 cases with PHC.Results：The count means of MV and MC in the cancer tissues were significantly higher than that in the peri-cancerous tissues(MV,67.30±13.68/HP vs.37.20±10.58 /HP,p<0.01;MC,15.64±4.54/HP vs 8.08±2.03 /HP,p<0.01);the count of MV in the patients with AFU(pre-operation) ≤10　μg/mL in serum(70.41±13.03) was significantly higher than that whose AFU >10 μg/mL(59.97±12.69)(p<0.05) and the count of MV in the patients with metastasis(73.50±13.77) was significantly higher than that of non-metastasis(64.10±12.68) (p<0.05).There were no correlation among the count of MV,MC and the other main clinicopathological features of PHC;the count of MC was significantly correlated with the count of MV（r=0.348，p<0.05）.Conclusions：The count of MC and MV might be closely related to the carcinogenesis and the progress of PHC;MC might be closely related to enhance the tumor angiogenesis.

Key words: primary hepatocarcinoma, microvessel, mast cell, count, immunohistochemistry

向志钢, 杨竹林, 钟飞. 原发性肝癌组织中MC与MV计数关系[J]. journal6, 2006, 27(5): 113-116.

XIANG Zhi-Gang, YANG Zhu-Lin, ZHONG Fei. Study on the Relationship Between the Counts of MC and MV in Tissues of Primary Hepatocarcinoma（PHC）[J]. journal6, 2006, 27(5): 113-116.

[1]	唐保祥, 任韩. 2类图1-因子数的计算公式[J]. 吉首大学学报（自然科学版）, 2021, 42(4): 1-3.
[2]	韩新风，许会芳，高海涛. Multisim软件在任意N进制计数器设计中的应用[J]. 吉首大学学报（自然科学版）, 2019, 40(6): 21-28.
[3]	陈仁辉, 范兆慧, 高辉. 大鼠DAI特殊染色观察及NF-κB的表达[J]. journal6, 2014, 35(5): 63-66.
[4]	龙述德. 带根近二正则平面地图的计数[J]. journal6, 2008, 29(3): 14-16.
[5]	龙述德, 王郡. 双奇异平面地图的数目[J]. journal6, 2008, 29(2): 7-9.
[6]	向志钢, 杨竹林, 钟飞. 原发性肝癌组织中TAM和MC与MV计数及病理意义[J]. journal6, 2007, 28(5): 120-124.