journal6 ›› 2003, Vol. 24 ›› Issue (3): 94-96.
• 科研简报 • 上一篇
出版日期:
发布日期:
作者简介:
Online:
Published:
摘要：利用反相液相色谱法（RP-HPLC）直接测定原料药中头孢拉定的含量.采用YQG-ODS色谱柱，以V(CH3OH）∶V(4%HAc-NaAc）＝3∶7的溶剂作流动相，于λ=262 nm处紫外检测，以峰面积外标法定量.头孢拉定在0.027～0.29 g·L-1浓度范围内具有良好的线性关系，回归方程为Y（μV·ｓ）=1.88×107X(g·L-1)+20 876,r=0.998 3，平均回收率为99.7%,RSD为0.57%（n=3）.
关键词： RP-HPLC, 原料药, 头孢拉定
Abstract: A RP-HPLC method for the determination of cefradin in raw drug was established.A column of YQG-ODS was used with a mobile phase of V(CH3OH）∶V(4%HAc-NaAc）＝3∶7 solution.The UV detector was set at 262 nm.The external standard method was used.There was a good linear correlation in the range of 0.027～0.29 g·L-1,Y（μV·ｓ）=1.88×107X(g·L-1)+20 876,r=0.998 3.The average recovery was 99.7%,RSD was 0.57%（n=3）.The elapsed time of a chromatographic analysis was 12 min.The method is rapid,accurate and reliable.
Key words: RP-HPLC, cefradin, external standard method
蒋剑波. RP-HPLC法测定原料药中头孢拉定的含量[J]. journal6, 2003, 24(3): 94-96.
JIANG Jian-Bo. Determination of Cefradin by RP-HPLC[J]. journal6, 2003, 24(3): 94-96.
0 / / 推荐
导出引用管理器 EndNote|Ris|BibTeX
链接本文: https://zkxb.jsu.edu.cn/CN/
https://zkxb.jsu.edu.cn/CN/Y2003/V24/I3/94
