Abstract: The concentration change and mechanism of glutamate at hyperxia induced retinal injury in newborn mice retinal injury are explored.The experimental group was put  in 95% hyperxia  environment to induce retinal damage,and the control groups in normal air.HE staining was used to observe retinal injury,ELIASA  to detecte the glutamate (Glu) concentration,and reverse transcription polymerase chain reaction(RT-PCR) to analyze the expression of retinal glutamate transporter (GLAST) and glutamine synthetase (GS) mRNA.The results showed that,compared with control group,hyperxia treatment group with retinal injury showed the increased  Glucontent,and decreased GLAST and GS mRNA expression.It thus can be concluded that hyperxia can lead to the increase of retinal glutamate in newborn mice,the mechanism of which was associated with decreased GLAST and GS mRNA expression.Glutamate increase may be one of the reasons for the hyperxia induced retinal injury.

Key words: hyperxia, glutamate, retina；newborn, mice